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Mouse Anti-CFLAR Recombinant Antibody (2F12) (CBMAB-C5061-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
2F12
Antibody Isotype
IgG2a, κ
Application
ELISA, ICC/IF, IHC-P, WB

Basic Information

Immunogen
CFLAR (178 a.a. ~ 277 a.a) partial recombinant protein.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)10 μg/ml
IHC3 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
CASP8 And FADD Like Apoptosis Regulator
Introduction
The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]
Entrez Gene ID
8837
UniProt ID
O15519
Alternative Names
CASP8 And FADD Like Apoptosis Regulator; Caspase-Like Apoptosis Regulatory Protein; CASP8 And FADD-Like Apoptosis Regulator; Cellular FLICE-Like Inhibitory Protein; FADD-Like Antiapoptotic Molecule 1; MACH-Related Inducer Of Toxicity; Caspase-Eight-Related Protein; Inhibitor Of FLICE; Caspase Homolog; CASP8AP1; FLAME-1; I-FLICE; Casper; C-FLIP;
Function
Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
Biological Process
Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: GO_Central
Apoptotic process Source: UniProtKB
Cellular response to dexamethasone stimulus Source: Ensembl
Cellular response to epidermal growth factor stimulus Source: Ensembl
Cellular response to estradiol stimulus Source: Ensembl
Cellular response to hypoxia Source: Ensembl
Cellular response to insulin stimulus Source: Ensembl
Cellular response to nitric oxide Source: Ensembl
Negative regulation of apoptotic process Source: ProtInc
Negative regulation of cardiac muscle cell apoptotic process Source: Ensembl
Negative regulation of cellular response to transforming growth factor beta stimulus Source: Ensembl
Negative regulation of extrinsic apoptotic signaling pathway Source: UniProtKB
Negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: MGI
Negative regulation of hepatocyte apoptotic process Source: Ensembl
Negative regulation of myoblast fusion Source: BHF-UCL
Negative regulation of reactive oxygen species biosynthetic process Source: Ensembl
Positive regulation of ERK1 and ERK2 cascade Source: Ensembl
Positive regulation of extracellular matrix organization Source: Ensembl
Positive regulation of glomerular mesangial cell proliferation Source: Ensembl
Positive regulation of hepatocyte proliferation Source: Ensembl
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Positive regulation of neuron projection development Source: Ensembl
Positive regulation of NF-kappaB transcription factor activity Source: BHF-UCL
Regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: Reactome
Regulation of necroptotic process Source: UniProtKB
Regulation of skeletal muscle satellite cell proliferation Source: BHF-UCL
Regulation of tumor necrosis factor-mediated signaling pathway Source: Reactome
Response to testosterone Source: Ensembl
Skeletal muscle atrophy Source: BHF-UCL
Skeletal muscle tissue development Source: BHF-UCL
Skeletal muscle tissue regeneration Source: BHF-UCL
Skeletal myofibril assembly Source: BHF-UCL
Viral process Source: UniProtKB-KW
Wound healing Source: BHF-UCL
Cellular Location
Cytosol; Ripoptosome; CD95 death-inducing signaling complex; Death-inducing signaling complex; Cytoplasm; Membrane raft
PTM
Proteolytically processed by CASP8 generating subunit p43 and p12.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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