Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-CFLAR Recombinant Antibody (CBXF-0115) (CBMAB-F0757-CQ)


See all CFLAR antibodies
Online Inquiry
Published Data
CBMAB-F0757-CQ-1
Mouse Anti-CFLAR Recombinant Antibody (CBXF-0115) (CBMAB-F0757-CQ) in IF
The expression level of c-FLIP after treatment with EGCG was significantly decreased. ...View More
Shahriari Felordi, M., Alikhani, M., Farzaneh, Z., Alipour Choshali, M., Ebrahimi, M., Aboulkheyr Es, H., ... & Vosough, M. (2023). (-)-Epigallocatechin-3-gallate induced apoptosis by dissociation of c-FLIP/Ku70 complex in gastric cancer cells. Journal of Cellular and Molecular Medicine, 27(17), 2572-2582.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-F0757-CQ-2
Mouse Anti-CFLAR Recombinant Antibody (CBXF-0115) (CBMAB-F0757-CQ) in WB
Protein expression of c-FLIP and Ku70 following treatment with EGCG after 24 h using western blot. ...View More
Shahriari Felordi, M., Alikhani, M., Farzaneh, Z., Alipour Choshali, M., Ebrahimi, M., Aboulkheyr Es, H., ... & Vosough, M. (2023). (-)-Epigallocatechin-3-gallate induced apoptosis by dissociation of c-FLIP/Ku70 complex in gastric cancer cells. Journal of Cellular and Molecular Medicine, 27(17), 2572-2582.
Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
CBMAB-F0757-CQ-3
Mouse Anti-CFLAR Recombinant Antibody (CBXF-0115) (CBMAB-F0757-CQ) in WB
The expression of FLIPL, FLIPS, caspase-8 and caspase-8 cleaved were analyzed by Western blot after treatment with δ-TT (20 μg/mL) from 1 h to 24 h in both DU145 and PC3 cells. Tubulin was used as loading control. Three independent experiments for each condition were analyzed; bar graph represents the mean optical density± SD. Statistical analysis was performed using one-way ANOVA followed by Dunnett's post-hoc test (** p < 0.01 vs. C; *** p < 0.001 vs. C). ...View More
Montagnani Marelli, M., Beretta, G., & Moretti, R. M. (2023). Necroptosis Induced by Delta-Tocotrienol Overcomes Docetaxel Chemoresistance in Prostate Cancer Cells. International Journal of Molecular Sciences, 24(5), 4923.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-F0757-CQ-4
Mouse Anti-CFLAR Recombinant Antibody (CBXF-0115) (CBMAB-F0757-CQ) in WB
WT and PLKEHO2−/− BMDMs were treated with TNFα (20ng/mL) and cycloheximide (CHX) (10μg/mL) for indicated periods of time. Cell lysates were probed with indicated antibodies. ...View More
Zhou, C., Zhang, X., Yang, C., He, Y., & Zhang, L. (2021). PLEKHO2 inhibits TNFα-induced cell death by suppressing RIPK1 activation. Cell Death & Disease, 12(8), 714.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBXF-0115
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1-202 of FLIPS/L of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CASP8 And FADD Like Apoptosis Regulator
Introduction
The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists.
Entrez Gene ID
8837
UniProt ID
O15519
Alternative Names
CASP8 And FADD Like Apoptosis Regulator; Caspase-Like Apoptosis Regulatory Protein; CASP8 And FADD-Like Apoptosis Regulator; Cellular FLICE-Like Inhibitory Protein; FADD-Like Antiapoptotic Molecule 1; MACH-Related Inducer Of Toxicity; Caspase-Eight-Related Protein; Inhibitor Of FLICE; Caspase Homolog; CASP8AP1; FLAME-1; I-FLICE; Casper; C-FLIP;
Function
Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
Biological Process
Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: GO_Central
Apoptotic process Source: UniProtKB
Cellular response to dexamethasone stimulus Source: Ensembl
Cellular response to epidermal growth factor stimulus Source: Ensembl
Cellular response to estradiol stimulus Source: Ensembl
Cellular response to hypoxia Source: Ensembl
Cellular response to insulin stimulus Source: Ensembl
Cellular response to nitric oxide Source: Ensembl
Negative regulation of apoptotic process Source: ProtInc
Negative regulation of cardiac muscle cell apoptotic process Source: Ensembl
Negative regulation of cellular response to transforming growth factor beta stimulus Source: Ensembl
Negative regulation of extrinsic apoptotic signaling pathway Source: UniProtKB
Negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: MGI
Negative regulation of hepatocyte apoptotic process Source: Ensembl
Negative regulation of myoblast fusion Source: BHF-UCL
Negative regulation of reactive oxygen species biosynthetic process Source: Ensembl
Positive regulation of ERK1 and ERK2 cascade Source: Ensembl
Positive regulation of extracellular matrix organization Source: Ensembl
Positive regulation of glomerular mesangial cell proliferation Source: Ensembl
Positive regulation of hepatocyte proliferation Source: Ensembl
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Positive regulation of neuron projection development Source: Ensembl
Positive regulation of NF-kappaB transcription factor activity Source: BHF-UCL
Regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: Reactome
Regulation of necroptotic process Source: UniProtKB
Regulation of skeletal muscle satellite cell proliferation Source: BHF-UCL
Regulation of tumor necrosis factor-mediated signaling pathway Source: Reactome
Response to testosterone Source: Ensembl
Skeletal muscle atrophy Source: BHF-UCL
Skeletal muscle tissue development Source: BHF-UCL
Skeletal muscle tissue regeneration Source: BHF-UCL
Skeletal myofibril assembly Source: BHF-UCL
Viral process Source: UniProtKB-KW
Wound healing Source: BHF-UCL
Cellular Location
Cytosol; Ripoptosome; CD95 death-inducing signaling complex; Death-inducing signaling complex; Cytoplasm; Membrane raft
PTM
Proteolytically processed by CASP8 generating subunit p43 and p12.
More Infomation
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-CFLAR Recombinant Antibody (CBXF-0115)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quoteOnline Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry