Antibody Array Analysis

Antibody arrays are valuable for the parallel analysis of multiple proteins in small sample volumes. The earliest and most widely used application of antibody arrays has been to measure multiple protein abundances, using sandwich assays and label-based assays, for biomarker discovery and biological studies. Modifications to these assays have led to studies profiling specific protein post-translational modifications, such as protein phosphorylation that is a mechanism of regulation that is extremely important in most cellular processes.

Fig.1 Cell signaling pathways^[2]

Identification and validation of protein expression patterns, characteristic of cancer progression, and tumor subtypes may intervene and improve tailored therapies in the clinical management of cancer patients. Antibody arrays are high-throughput tools that improve the functional characterization of molecular bases for disease. Creative Biolabs has a wide range of antibody arrays and can be used to study protein changes and phosphorylation profiling in cellular signaling pathways. Futhermore, we offer antibody array analysis service which allows customers to send samples to our lab and get them analyzed with our antibody arrays. Scanned images on our microarray scanners will be delivered to you by email within 3-4 weeks.

Fig. 2 The basic flow of antibody array/protein array analysis^[1].

Protein Phosphorylation Antibody Array Analysis

Cytokine Antibody Array Analysis

For more detailed information about our antibody arrays, please feel free to contact us.

References

1. Lueong, S.; et al. Protein microarrays as tools for functional proteomics: achievements, promises and challenges. J Proteomics & Bioinform. 2013, 7, 004.
2. Kingsmore, S. F.; Multiplexed protein measurement: technologies and applications of protein and antibody arrays. Nature reviews Drug discovery. 2006, 5(4), 310-321.