Glass Slide Antibody Arrays

Fig.1 Schematics of workflow¹.

Antibody arrays have emerged as an important tool, which enable multiplexed protein expression profiling such as cytokines, chemokines and growth factors, which may be involved in diseases. To design high-performing antibody arrays, it is necessary to develop an integrated technology platform, where the interplay between the surfaces and the antibody is anticipated to play an important role.

Creative Biolabs Glass Slide Antibody Arrays were introduced as a new array platform. Chemically derivatized glass slides allow for printing of sub-microliter sized antibody spots, reducing the array surface area without sacrificing spot density. In contrast to the chemiluminescent membrane arrays, glass slide-based antibody arrays reduce the volume of sample consumed and can also be easily fitted to high-throughput liquid handling systems owing to their smooth and rigid structure. The glass slide antibody arrays also utilize a fluorescent signal readout and have a wider dynamic detection range than by chemiluminescence.

Glass Slide Antibody Arrays Workflow

• Print capture antibodies in glass slide
• Block glass slide array with buffer
• Incubate protein samples (as little as 10 µl)
• Wash off nonspecific proteins
• Incubate a cocktail of biotinylated detection antibodies
• Incubate streptavidin-conjugated fluorescence
• Visualize signals by fluorescence laser scanner

Please contact Creative Biolabs for glass slide antibody arrays technology and related products.

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