Antibody arrays are valuable for the parallel analysis of multiple proteins in small sample volumes. The widely used application of antibody arrays has been for biomarker discovery and biological studies. Analogous to ELISA, antibody arrays are an antibody-pair-based assay. The first array approaches attempted to miniaturize biochemical and immunobiological assays performed in 96-well plates. But the small surface area in each well limits the number of antibody spots while 96-well plate-based antibody arrays have high-throughput capability. Nitrocellulose membranes and glass slides were subsequently utilized to develop arrays which could accommodate larger panels of antibodies. Nitrocellulose membrane-based arrays are flexible, easy to handle, and have increased protein binding capacity.
Fig.1 Schematic representation of the antibody
array experimental formats.
Creative Biolabs Membrane Antibody Arrays are used to measure many proteins simultaneously in one experiment, with significantly less time, effort and cost than ELISA or western blot assays. Moreover, membrane-based antibody arrays are very easy to set up and significantly cheaper compared to other multiplex immunoassays.
Membrane Antibody Arrays Workflow
Please contact Creative Biolabs for membrane antibody arrays technology and related products.
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