Mouse Anti-CAPNS1 Monoclonal Antibody (1D11) (CBMAB-0951-YC)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1D11

Antibody Isotype

IgG1, κ

Application

ELISA, WB, IF

Basic Information

Immunogen

Recombinant human CAPNS1 (aa 84-268) purified from E. coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IF(ICC)	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4, 10% Glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Purity

>95%, as determined by SDS-PAGE analysis

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

calpain, small subunit 1

Introduction

Calpains operate as heterodimers, comprising a specific large catalytic subunit (calpain 1 subunit in Calpain I, and calpain 2 subunit in Calpain II), and a common small regulatory subunit encoded by this gene. CAPNS1 is a member of the calpain small subunit family, which is essential for the stability and function of both calpain heterodimers. The proteolytic activities of CAPNS1 influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy.

Entrez Gene ID

Human	826
Mouse	12336

UniProt ID

Human	P04632
Mouse	O88456

Alternative Names

CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4

Function

Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Biological Process

Cornification Source: Reactome
Extracellular matrix disassembly Source: Reactome
Positive regulation of cell population proliferation Source: ProtInc
Regulation of macroautophagy Source: ParkinsonsUK-UCL

Cellular Location

Cytoplasm; Cell membrane. Translocates to the plasma membrane upon calcium binding.

More Infomation

References

Wang, J., Ciampa, G., Zheng, D., Shi, Q., Chen, B., Abel, E. D., ... & Song, L. S. (2021). Calpain-2 Specifically Cleaves Junctophilin-2 at the Same Site as Calpain-1 but with Less Efficacy. Biochemical Journal.

Zhuang, Q., Fan, M., Shen, J., Chen, Z., Xue, D., Lu, H., ... & Hou, J. (2021). Overexpression of Capns1 Predicts Poor Prognosis and Correlates with Tumor Progression in Renal Cell Carcinoma. Urologia internationalis, 1-8.

Tang, S., Yin, Q., Liu, F., & Zhang, Y. (2019). Calpain Small Subunit 1 Protein in the Prognosis of Cancer Survivors and Its Clinicopathological Correlation. BioMed research international, 2019.

MacLeod, J. A., Gao, Y., Hall, C., Muller, W. J., Gujral, T. S., & Greer, P. A. (2018). Genetic disruption of calpain-1 and calpain-2 attenuates tumorigenesis in mouse models of HER2+ breast cancer and sensitizes cancer cells to doxorubicin and lapatinib. Oncotarget, 9(70), 33382.

Grochowska, E., Borys, B., Grześkowiak, E., & Mroczkowski, S. (2017). Effect of the calpain small subunit 1 gene (CAPNS1) polymorphism on meat quality traits in sheep. Small Ruminant Research, 150, 15-21.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

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Mouse Anti-CAPNS1 Recombinant Antibody (156)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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