Mouse Anti-CAPNS1 Recombinant Antibody (156) (CBMAB-C3217-LY)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Cattle, Human

Clone

156

Antibody Isotype

IgG1

Application

IF, WB

Basic Information

Immunogen

Purified bovine skeletal muscle 28 kDa calpain subunit.

Host Species

Mouse

Specificity

Cattle, Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IF(ICC)	1:10-1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Buffer

PBS

Preservative

0.05% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

calpain, small subunit 1

Introduction

CAPNS1 (Calpain Small Subunit 1) is a Protein Coding gene. Diseases associated with CAPNS1 include Chronic Neutrophilic Leukemia. Among its related pathways are Apoptosis Modulation and Signaling and Keratinization. Gene Ontology (GO) annotations related to this gene include calcium ion binding and calcium-dependent cysteine-type endopeptidase activity.
An important paralog of this gene is CAPNS2.

Entrez Gene ID

Human	826
Dog	611746

UniProt ID

Human	P04632
Dog	J9NT23

Function

Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Biological Process

Cornification Source: Reactome
Extracellular matrix disassembly Source: Reactome
Positive regulation of cell population proliferation Source: ProtInc
Regulation of macroautophagy Source: ParkinsonsUK-UCL

Cellular Location

Cytoplasm; Cell membrane. Translocates to the plasma membrane upon calcium binding.

More Infomation

References

Wang, J., Ciampa, G., Zheng, D., Shi, Q., Chen, B., Abel, E. D., ... & Song, L. S. (2021). Calpain-2 Specifically Cleaves Junctophilin-2 at the Same Site as Calpain-1 but with Less Efficacy. Biochemical Journal.

Zhuang, Q., Fan, M., Shen, J., Chen, Z., Xue, D., Lu, H., ... & Hou, J. (2021). Overexpression of Capns1 Predicts Poor Prognosis and Correlates with Tumor Progression in Renal Cell Carcinoma. Urologia internationalis, 1-8.

Tang, S., Yin, Q., Liu, F., & Zhang, Y. (2019). Calpain Small Subunit 1 Protein in the Prognosis of Cancer Survivors and Its Clinicopathological Correlation. BioMed research international, 2019.

MacLeod, J. A., Gao, Y., Hall, C., Muller, W. J., Gujral, T. S., & Greer, P. A. (2018). Genetic disruption of calpain-1 and calpain-2 attenuates tumorigenesis in mouse models of HER2+ breast cancer and sensitizes cancer cells to doxorubicin and lapatinib. Oncotarget, 9(70), 33382.

Grochowska, E., Borys, B., Grześkowiak, E., & Mroczkowski, S. (2017). Effect of the calpain small subunit 1 gene (CAPNS1) polymorphism on meat quality traits in sheep. Small Ruminant Research, 150, 15-21.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-CAPNS1 Monoclonal Antibody (1D11)

Mouse Anti-CAPNS1 Recombinant Antibody (CBLNC-140)

Rabbit Anti-CAPNS1 Recombinant Antibody (C9695)

Rabbit Anti-CAPNS1 Recombinant Antibody (CBFYC-0803)

Mouse Anti-CAPNS1 Recombinant Antibody (CBFYC-0802)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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Second antibody and isotype control

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