Mouse Anti-CA8 Recombinant Antibody (CBFYC-0701) (CBMAB-C0755-FY)

See all CA8 antibodies

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Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CBFYC-0701

Antibody Isotype

IgG2a, κ

Application

E, WB, IF, IP

Basic Information

Immunogen

Amino acids 1-100 mapping at the N-terminus of CA VIII of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-290

Target

Full Name

CA8

Introduction

The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes.

Entrez Gene ID

767

UniProt ID

P35219

Alternative Names

CALS; CARP; CA-RP; CAMRQ3; CA-VIII

Function

Does not have a carbonic anhydrase catalytic activity.

Biological Process

One-carbon metabolic process Source: GO_Central
Phosphatidylinositol-mediated signaling Source: Ensembl

Cellular Location

Cytoplasm

Involvement in disease

Cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CMARQ3): A congenital cerebellar ataxia associated with dysarthia, quadrupedal gait and mental retardation.

More Infomation

References

Chi, T. H., Hsieh, B. Y., Liang, P. S., Han, T. H., & Hsieh, M. (2021). Expression and Functional Study of Single Mutations of Carbonic Anhydrase 8 in Neuronal Cells. Cellular and Molecular Neurobiology, 41(6), 1355-1371.

Mamoor, S. (2021). Carbonic anhydrase VIII (CA8) is a differentially expressed gene in brain metastatic human breast cancer.

Paternoster, L., Soblet, J., Aeby, A., De Tiège, X., Goldman, S., Yue, W. W., ... & Deconinck, N. (2020). Novel homozygous variant of carbonic anhydrase 8 gene expanding the phenotype of cerebellar ataxia, mental retardation, and disequilibrium syndrome subtype 3. American Journal of Medical Genetics Part A, 182(11), 2685-2693.

Sanyanga, T. A., & Tastan Bishop, Ö. (2020). Structural characterization of carbonic anhydrase VIII and effects of missense single nucleotide variations to protein structure and function. International journal of molecular sciences, 21(8), 2764.

Hsieh, M., Hsieh, B. Y., Ma, C. Y., Li, Y. T., Liu, C. S., & Lo, C. M. (2019). Protective roles of carbonic anhydrase 8 in Machado–Joseph Disease. Journal of neuroscience research, 97(10), 1278-1297.

Lo, C. M., Ma, Y. S., Wei, Y. H., Hsieh, B. Y., & Hsieh, M. (2018). Promoter analysis and transcriptional regulation of human carbonic anhydrase VIII gene in a MERRF disease cell model. Archives of biochemistry and biophysics, 641, 50-61.

Zhuang, G. Z., Upadhyay, U., Tong, X., Kang, Y., Erasso, D. M., Fu, E. S., ... & Levitt, R. C. (2018). Human carbonic anhydrase-8 AAV8 gene therapy inhibits nerve growth factor signaling producing prolonged analgesia and anti-hyperalgesia in mice. Gene therapy, 25(4), 297-311.

Paternoster, L., Soblet, J., Aeby, A., Vilain, C., Smits, G., & Deconinck, N. (2017). A new mutation of carbonic anhydrase 8 gene expanding the cerebellar ataxia, mental retardation and disequilibrium syndrome (CAMRQ) subtype 3. J Neurol Sci, 381, 1136-7.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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