Mouse Anti-MYH1 Recombinant Antibody (CBXS-5905) (CBMAB-S0291-CQ)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat, Chicken, Rabbit

Clone

CBXS-5905

Antibody Isotype

IgG1

Application

WB, IHC

Basic Information

Immunogen

Purified human skeletal muscle myosin

Specificity

Human, Mouse, Rat, Chicken, Rabbit

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

myosin heavy chain 1

Introduction

Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. This protein plays a role of muscle contraction.

Entrez Gene ID

Human	4619
Mouse	17879
Rat	287408
Rabbit	100125991

UniProt ID

Human	P12882
Mouse	Q5SX40
Rat	F1LRV9
Rabbit	A0A5F9CQ93

Alternative Names

HEL71; MYHSA1; MYHa; MyHC-2X/D; MyHC-2x

Function

Muscle contraction.

Biological Process

Muscle contraction Source: Ensembl

Cellular Location

Other locations
myofibril
Note: Thick filaments of the myofibrils.

More Infomation

References

Hau, H. T. A., Kelu, J. J., Ochala, J., & Hughes, S. M. (2023). Slow myosin heavy chain 1 is required for slow myofibril and muscle fibre growth but not for myofibril initiation. Developmental Biology, 499, 47-58.

Long, K., Su, D., Li, X., Li, H., Zeng, S., Zhang, Y., ... & Li, M. (2022). Identification of enhancers responsible for the coordinated expression of myosin heavy chain isoforms in skeletal muscle. BMC genomics, 23(1), 1-13.

Valberg, S. J., Schultz, A. E., Finno, C. J., Bellone, R. R., & Hughes, S. S. (2022). Prevalence of clinical signs and factors impacting expression of myosin heavy chain myopathy in Quarter Horse‐related breeds with the MYH1 E321G mutation. Journal of Veterinary Internal Medicine, 36(3), 1152-1159.

Ochala, J., Finno, C. J., & Valberg, S. J. (2021). Myofibre hyper-contractility in horses expressing the myosin heavy chain myopathy mutation, MYH1E321G. Cells, 10(12), 3428.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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Second antibody and isotype control

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