Mouse Anti-GRHL1 Antibody (1B6) (CBMAB-0417-YC)

See all GRHL1 antibodies

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1B6

Antibody Isotype

IgG1

Application

IP, WB

Basic Information

Immunogen

Recombinant peptide

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

GRHL1

Introduction

GRHL1 (grainyhead-like protein 1) is a member of the grainyhead family of transcription factors and exist as a homodimer or can form heterodimers with sister-of-mammalian grainyhead or brother-of-mammalian grainyhead. GRHL1 functions as a transcription factor during development.

Entrez Gene ID

29841

UniProt ID

Q9NZI5

Alternative Names

LBP-32; MGR; TFCP2L2

Function

Transcription factor involved in epithelial development. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' (PubMed:12175488, PubMed:18288204, PubMed:29309642).

Important regulator of DSG1 in the context of hair anchorage and epidermal differentiation, participates in the maintenance of the skin barrier. There is no genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity during epithelia development (By similarity).

Isoform 1:
Functions as transcription activator.

Isoform 2:
May function as a repressor in tissues where both isoform 1 and isoform 2 are expressed.

Biological Process

Desmosome organization Source: Ensembl
Epidermis development Source: UniProtKB
Establishment of skin barrier Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of keratinocyte differentiation Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Nucleus

More Infomation

References

Proft, S., Leiz, J., Heinemann, U., Seelow, D., Schmidt-Ott, K. M., & Rutkiewicz, M. (2022). Discovery of a non-canonical GRHL1 binding motif using deep convolutional and recurrent neural networks. bioRxiv, 2022-06.

He, Y., Gan, M., Wang, Y., Huang, T., Wang, J., Han, T., & Yu, B. (2021). EGFR-ERK induced activation of GRHL1 promotes cell cycle progression by up-regulating cell cycle related genes in lung cancer. Cell Death & Disease, 12(5), 430.

Li, M., Li, Z., Guan, X., & Qin, Y. (2019). Suppressor gene GRHL1 is associated with prognosis in patients with oesophageal squamous cell carcinoma. Oncology Letters, 17(5), 4313-4320.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-GRHL1 Recombinant Antibody (CBLG1-3206)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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