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Mouse Anti-FADD Recombinant Antibody (CBXF-2818) (CBMAB-F3528-CQ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXF-2818
Antibody Isotype
IgG1, λ
Application
ELISA, WB

Basic Information

Immunogen
Partial recombinant corresponding to aa109-208 from FADD (AAH00334) with GST tag
Specificity
Human
Antibody Isotype
IgG1, λ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Fas Associated Via Death Domain
Introduction
The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.
Entrez Gene ID
8772
UniProt ID
Q13158
Alternative Names
Fas Associated Via Death Domain; Fas-Associating Death Domain-Containing Protein; Growth-Inhibiting Gene 3 Protein; Fas-Associating Protein With Death Domain; Fas (TNFRSF6)-Associated Via Death Domain; Mediator Of Receptor-Induced Toxicity;
Research Area
Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors (PubMed:7538907, PubMed:23955153, PubMed:19118384, PubMed:20935634, PubMed:16762833, PubMed:24025841).

The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation (PubMed:7538907, PubMed:19118384, PubMed:20935634, PubMed:16762833).

Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis (PubMed:16762833).

Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling (PubMed:21109225).
Biological Process
Activation of cysteine-type endopeptidase activity Source: BHF-UCL
Apoptotic process Source: UniProtKB
Apoptotic signaling pathway Source: BHF-UCL
Behavioral response to cocaine Source: Ensembl
Cellular response to mechanical stimulus Source: UniProtKB
Death-inducing signaling complex assembly Source: UniProtKB
Defense response to virus Source: UniProtKB
Extrinsic apoptotic signaling pathway Source: UniProtKB
Extrinsic apoptotic signaling pathway in absence of ligand Source: Ensembl
Extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
Innate immune response Source: UniProtKB-KW
Kidney development Source: Ensembl
Lymph node development Source: UniProtKB
Motor neuron apoptotic process Source: Ensembl
Necroptotic signaling pathway Source: BHF-UCL
Negative regulation of activation-induced cell death of T cells Source: UniProtKB
Negative regulation of necroptotic process Source: Ensembl
Positive regulation of activated T cell proliferation Source: UniProtKB
Positive regulation of adaptive immune response Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of CD8-positive, alpha-beta cytotoxic T cell extravasation Source: UniProtKB
Positive regulation of extrinsic apoptotic signaling pathway Source: UniProtKB
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Positive regulation of interferon-gamma production Source: UniProtKB
Positive regulation of interleukin-8 production Source: BHF-UCL
Positive regulation of macrophage differentiation Source: UniProtKB
Positive regulation of proteolysis Source: BHF-UCL
Positive regulation of T cell mediated cytotoxicity Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of tumor necrosis factor production Source: BHF-UCL
Positive regulation of type I interferon-mediated signaling pathway Source: UniProtKB
Response to morphine Source: Ensembl
Spleen development Source: UniProtKB
T cell differentiation in thymus Source: UniProtKB
T cell homeostasis Source: UniProtKB
Thymus development Source: UniProtKB
TRAIL-activated apoptotic signaling pathway Source: ParkinsonsUK-UCL
Cellular Location
Cytosol; Ripoptosome; Nucleus; CD95 death-inducing signaling complex; Death-inducing signaling complex; Plasma membrane; Cell body; Cytoplasm; Membrane raft; Neuron projection
Involvement in disease
Infections, recurrent, associated with encephalopathy, hepatic dysfunction and cardiovascular malformations (IEHDCM):
A condition with biological features of autoimmune lymphoproliferative syndrome such as high-circulating CD4(-)CD8(-)TCR-alpha-beta(+) T-cell counts, and elevated IL10 and FASL levels. Affected individuals suffer from recurrent, stereotypical episodes of fever, encephalopathy, and mild liver dysfunction sometimes accompanied by generalized seizures. The episodes can be triggered by varicella zoster virus (VZV), measles mumps rubella (MMR) attenuated vaccine, parainfluenza virus, and Epstein-Barr virus (EBV).
PTM
(Microbial infection) Glycosylated at Arg-117 by enteropathogenic E.coli protein NleB1, C.rodentium protein NleB and S.typhimurium protein Ssek1: arginine GlcNAcylation prevents recruitment of caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors.
More Infomation

He, X., Zhang, L., Hu, L., Liu, S., Xiong, A., Wang, J., ... & Li, G. (2021). PM2. 5 Aggravated OVA-Induced Epithelial Tight Junction Disruption Through Fas Associated via Death Domain-Dependent Apoptosis in Asthmatic Mice. Journal of Asthma and Allergy, 14, 1411.

Xu, Z., Zheng, M., Liu, Y., Zhang, L., Zhang, X., & Bai, R. (2021). Roles of TNF receptor-associated and Fas-associated death domain proteins in the apoptosis of Eimeria tenella host cells. Veterinary Parasitology, 290, 109351.

Zhao, Y., Guo, M., Lv, Z., Zhang, W., Shao, Y., Zhao, X., & Li, C. (2020). Fas-associated death domain (FADD) in sea cucumber (Apostichopus japonicus): Molecular cloning, characterization and pro-apoptotic function analysis. Developmental & Comparative Immunology, 108, 103673.

Hollomon, M. G., Patterson, L., Santiago-O'Farrill, J., Kleinerman, E. S., & Gordon, N. (2020). Knock down of Fas-associated protein with death domain (FADD) sensitizes osteosarcoma to TNFα-induced cell death. Journal of Cancer, 11(7), 1657.

Koutsogiannaki, S., Hou, L., Babazada, H., Okuno, T., Blazon-Brown, N., Soriano, S. G., ... & Yuki, K. (2019). The volatile anesthetic sevoflurane reduces neutrophil apoptosis via Fas death domain–Fas-associated death domain interaction. The FASEB Journal, 33(11), 12668.

Mouasni, S., & Tourneur, L. (2018). FADD at the Crossroads between Cancer and Inflammation. Trends in immunology, 39(12), 1036-1053.

Muhammad, I. F., Borné, Y., Melander, O., Orho-Melander, M., Nilsson, J., Söderholm, M., & Engström, G. (2018). FADD (Fas-associated protein with death domain), caspase-3, and caspase-8 and incidence of ischemic stroke. Stroke, 49(9), 2224-2226.

Peng, M., Wang, Y., Qiang, L., Xu, Y., Li, C., Li, T., ... & Wang, J. (2018). Interleukin-35 inhibits TNF-α-induced osteoclastogenesis and promotes apoptosis via shifting the activation from tnf receptor-associated death domain (TRADD)–TRAF2 to TRADD–Fas-associated death domain by JAK1/STAT1. Frontiers in immunology, 9, 1417.

Hernández-Hernández, E., Miralles, A., Esteban, S., & García-Fuster, M. J. (2018). Repeated treatment with the α2-adrenoceptor agonist UK-14304 improves cognitive performance in middle-age rats: role of hippocampal Fas-associated death domain. Journal of Psychopharmacology, 32(2), 248-255.

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For research use only. Not intended for any clinical use.

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