Mouse Anti-ELF2 Antibody (5A5) (CBMAB-0349-YC)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

5A5

Antibody Isotype

IgG2b

Application

IP, WB, MA

Basic Information

Immunogen

Recombinant protein

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

ELF2

Introduction

ELF2 (E74-like factor 2 (ets domain transcription factor)), formerly known as new Ets-related factor (NERF), is an ETS family transcription factor. In humans this protein is encoded by the ELF2 gene.

Entrez Gene ID

1998

UniProt ID

Q15723

Alternative Names

EU32; NERF; NERF-1A; NERF-1B; NERF-2

Research Area

Isoform 1 transcriptionally activates the LYN and BLK promoters and acts synergistically with RUNX1 to transactivate the BLK promoter.

Isoform 2 may function in repression of RUNX1-mediated transactivation.

Biological Process

Cell differentiation Source: GO_Central
Negative regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: UniProtKB

Cellular Location

Nucleus

More Infomation

References

Qi, X., Chen, D., Yu, W., Wang, L., Liu, L., & Tao, X. (2022). Long non-coding RNA PRNCR1 promotes ovarian cancer cell proliferation, migration and invasion by targeting the miR-653-5p/ELF2 axis. Molecular and Cellular Biochemistry, 477(5), 1463-1475.

Ma, X., & Li, Y. (2022). Circ_0028007 Aggravates the Malignancy of Nasopharyngeal Carcinoma by Regulating miR-656-3p/ELF2 Axis. Biochemical Genetics, 1-18.

Li, M., Li, Y., & Yu, M. (2020). CircRNA ZNF609 knockdown suppresses cell growth via modulating miR-188/ELF2 axis in nasopharyngeal carcinoma. OncoTargets and therapy, 13, 2399.

Zhang, W., Xu, C., Guo, J., Guo, L., Huo, H., & Wang, H. (2020). Circ-ELF2 acts as a competing endogenous RNA to facilitate glioma cell proliferation and aggressiveness by targeting MiR-510-5p/MUC15 signaling. OncoTargets and therapy, 13, 10087.

Jia, C. C., Du, J., Liu, X., Jiang, R., Huang, Y., Wang, T., ... & Wang, B. (2018). B-cell receptor-associated protein 31 regulates the expression of valosin-containing protein through Elf2. Cellular Physiology and Biochemistry, 51(4), 1799-1814.

Ahmad, H., Requena, T., Frejo, L., Cobo, M., Gallego-Martinez, A., Martin, F., ... & Bronstein, A. M. (2018). Clinical and functional characterization of a missense ELF2 variant in a CANVAS family. Frontiers in genetics, 9, 85.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-ELF2 Antibody (1B5)

Mouse Anti-ELF2 Antibody (1F1)

Mouse Anti-ELF2 Recombinant Antibody (CBFYE-0768)

Mouse Anti-ELF2 Recombinant Antibody (CBFYE-0766)

Mouse Anti-ELF2 Antibody (3H4)

Mouse Anti-ELF2 Antibody (3A9)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Second antibody and isotype control

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