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Mouse Anti-EBF1 Recombinant Antibody (1G8) (CBMAB-A2424-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
1G8
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
EBF1 (AAH38805, 1 a.a. ~ 591 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
EBF1
Entrez Gene ID
1879
UniProt ID
Q9UH73
Alternative Names
COE1; EBF; FLJ39389; FLJ41763; O/E-1; OLF1
Research Area
Key pioneer transcription factor of B-cell specification and commitment (PubMed:27807034).

Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Operates in a transcription factor network to activate B-cell-specific genes and repress genes associated with alternative cell fates. For instance, positively regulates many B-cell specific genes including BCR or CD40 while repressing genes that direct cells into alternative lineages, including GATA3 and TCF7 for the T-cell lineage. In addition to its role during lymphopoiesis, controls the thermogenic gene program in adipocytes during development and in response to environmental cold (By similarity).

(Microbial infection) Acts as a chromatin anchor for Epstein-Barr virus EBNA2 to mediate the assembly of EBNA2 chromatin complexes in B-cells (PubMed:28968461).

In addition, binds to the viral LMP1 proximal promoter and promotes its expression during latency (PubMed:26819314).
Biological Process
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus
More Infomation

Shuang, O., Zhou, J., Cai, Z., Liao, L., Wang, Y., Wang, W., & Xu, M. (2022). EBF1-mediated up-regulation of lncRNA FGD5-AS1 facilitates osteosarcoma progression by regulating miR-124-3p/G3BP2 axis as a ceRNA. Journal of Orthopaedic Surgery and Research, 17(1), 1-14.

Qiu, Z., Guo, W., Dong, B., Wang, Y., Deng, P., Wang, C., ... & Wu, Y. (2022). EBF1 promotes triple-negative breast cancer progression by surveillance of the HIF1α pathway. Proceedings of the National Academy of Sciences, 119(28), e2119518119.

Pagani, F., Tratta, E., Dell’Era, P., Cominelli, M., & Poliani, P. L. (2021). EBF1 is expressed in pericytes and contributes to pericyte cell commitment. Histochemistry and cell biology, 156(4), 333-347.

El-Magd, M. A., Abdelfattah-Hassan, A., Elsisy, R. A., Hawsawi, Y. M., Oyouni, A. A., Al-Amer, O. M., & El-Shetry, E. S. (2021). Expression and function of Ebf1 gene during chondrogenesis in chick embryo limb buds. Gene, 803, 145895.

Somasundaram, R., Jensen, C. T., Tingvall-Gustafsson, J., Åhsberg, J., Okuyama, K., Prasad, M., ... & Sigvardsson, M. (2021). EBF1 and PAX5 control pro-B cell expansion via opposing regulation of the Myc gene. Blood, 137(22), 3037-3049.

Luo, H., Yang, L., Liu, C., Wang, X., Dong, Q., Liu, L., & Wei, Q. (2020). TMPO-AS1/miR-98-5p/EBF1 feedback loop contributes to the progression of bladder cancer. The International Journal of Biochemistry & Cell Biology, 122, 105702.

Wang, Y., Zolotarev, N., Yang, C. Y., Rambold, A., Mittler, G., & Grosschedl, R. (2020). A prion-like domain in transcription factor EBF1 promotes phase separation and enables B cell programming of progenitor chromatin. Immunity, 53(6), 1151-1167.

Nelson, T., Velazquez, H., Troiano, N., & Fretz, J. A. (2019). Early B cell factor 1 (EBF1) regulates glomerular development by controlling mesangial maturation and consequently COX-2 expression. Journal of the American Society of Nephrology, 30(9), 1559-1572.

Boller, S., Li, R., & Grosschedl, R. (2018). Defining B cell chromatin: lessons from EBF1. Trends in Genetics, 34(4), 257-269.

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For research use only. Not intended for any clinical use.

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