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Mouse Anti-EBF1 Antibody (1B1) (CBMAB-0329-YC)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
1B1
Antibody Isotype
IgG2c
Application
IP

Basic Information

Immunogen
Protein domain
Specificity
Human
Antibody Isotype
IgG2c
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
EBF1
Introduction
EBF1 (early B-cell factor 1) is a protein that has transcription factor activity, sequence-specific DNA binding and C2H2 zinc finger domain binding. Diseases associated with EBF1 include fetal erythroblastosis and hematologic cancer. Among its related pathways are developmental biology and adipogenesis.
Entrez Gene ID
1879
UniProt ID
Q9UH73
Alternative Names
Early B-Cell Factor 1; Early B-Cell Factor; O/E-1; COE1; EBF; Collier, Olf And EBF Transcription Factor 1; Olfactory Neuronal Transcription Factor 1; OLF1; OE-1
Research Area
Key pioneer transcription factor of B-cell specification and commitment (PubMed:27807034).

Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Operates in a transcription factor network to activate B-cell-specific genes and repress genes associated with alternative cell fates. For instance, positively regulates many B-cell specific genes including BCR or CD40 while repressing genes that direct cells into alternative lineages, including GATA3 and TCF7 for the T-cell lineage. In addition to its role during lymphopoiesis, controls the thermogenic gene program in adipocytes during development and in response to environmental cold (By similarity).

(Microbial infection) Acts as a chromatin anchor for Epstein-Barr virus EBNA2 to mediate the assembly of EBNA2 chromatin complexes in B-cells (PubMed:28968461).

In addition, binds to the viral LMP1 proximal promoter and promotes its expression during latency (PubMed:26819314).
Biological Process
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus
More Infomation

Shuang, O., Zhou, J., Cai, Z., Liao, L., Wang, Y., Wang, W., & Xu, M. (2022). EBF1-mediated up-regulation of lncRNA FGD5-AS1 facilitates osteosarcoma progression by regulating miR-124-3p/G3BP2 axis as a ceRNA. Journal of Orthopaedic Surgery and Research, 17(1), 1-14.

Qiu, Z., Guo, W., Dong, B., Wang, Y., Deng, P., Wang, C., ... & Wu, Y. (2022). EBF1 promotes triple-negative breast cancer progression by surveillance of the HIF1α pathway. Proceedings of the National Academy of Sciences, 119(28), e2119518119.

Pagani, F., Tratta, E., Dell’Era, P., Cominelli, M., & Poliani, P. L. (2021). EBF1 is expressed in pericytes and contributes to pericyte cell commitment. Histochemistry and cell biology, 156(4), 333-347.

El-Magd, M. A., Abdelfattah-Hassan, A., Elsisy, R. A., Hawsawi, Y. M., Oyouni, A. A., Al-Amer, O. M., & El-Shetry, E. S. (2021). Expression and function of Ebf1 gene during chondrogenesis in chick embryo limb buds. Gene, 803, 145895.

Somasundaram, R., Jensen, C. T., Tingvall-Gustafsson, J., Åhsberg, J., Okuyama, K., Prasad, M., ... & Sigvardsson, M. (2021). EBF1 and PAX5 control pro-B cell expansion via opposing regulation of the Myc gene. Blood, 137(22), 3037-3049.

Luo, H., Yang, L., Liu, C., Wang, X., Dong, Q., Liu, L., & Wei, Q. (2020). TMPO-AS1/miR-98-5p/EBF1 feedback loop contributes to the progression of bladder cancer. The International Journal of Biochemistry & Cell Biology, 122, 105702.

Wang, Y., Zolotarev, N., Yang, C. Y., Rambold, A., Mittler, G., & Grosschedl, R. (2020). A prion-like domain in transcription factor EBF1 promotes phase separation and enables B cell programming of progenitor chromatin. Immunity, 53(6), 1151-1167.

Nelson, T., Velazquez, H., Troiano, N., & Fretz, J. A. (2019). Early B cell factor 1 (EBF1) regulates glomerular development by controlling mesangial maturation and consequently COX-2 expression. Journal of the American Society of Nephrology, 30(9), 1559-1572.

Boller, S., Li, R., & Grosschedl, R. (2018). Defining B cell chromatin: lessons from EBF1. Trends in Genetics, 34(4), 257-269.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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