Rat Anti-CLTA Recombinant Antibody (CBYY-C0463) (CBMAB-C1420-YY)

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Datasheet

Summary

Host Animal

Rat

Specificity

Mouse

Clone

CBYY-C0463

Antibody Isotype

IgG2a, κ

Application

FC, IP

Basic Information

Specificity

Mouse

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

0.5 mg/mL

Preservative

PBS

Concentration

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

clathrin, light chain (Lca)

Entrez Gene ID

12757

UniProt ID

O08585

Alternative Names

CD1b Molecule; CD1B Antigen, B Polypeptide; CD1b Antigen; Differentiation Antigen CD1-Alpha-3; T-Cell Surface Glycoprotein CD1b; Cortical Thymocyte Antigen CD1B; CD1A; CD1; R1;

Function

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:21297582).

Biological Process

Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Clathrin coat assembly Source: CAFA
Clathrin-dependent endocytosis Source: GO_Central
Intracellular protein transport Source: InterPro
Low-density lipoprotein particle clearance Source: Reactome
Low-density lipoprotein particle receptor catabolic process Source: Reactome
Membrane organization Source: Reactome
Post-Golgi vesicle-mediated transport Source: Reactome

Cellular Location

Spindle; Cytoplasmic vesicle membrane; Coated pit. Cytoplasmic face of coated pits and vesicles. In complex with TACC3 and CKAP5 (forming the TACC3/ch-TOG/clathrin complex) localized to inter-microtubule bridges in mitotic spindles.

More Infomation

References

Redlingshöfer, L., McLeod, F., Chen, Y., Camus, M. D., Burden, J. J., Palomer, E., ... & Brodsky, F. M. (2020). Clathrin light chain diversity regulates membrane deformation in vitro and synaptic vesicle formation in vivo. Proceedings of the National Academy of Sciences, 117(38), 23527-23538.

Tsygankova, O. M., & Keen, J. H. (2019). A unique role for clathrin light chain A in cell spreading and migration. Journal of cell science, 132(10), jcs224030.

Biancospino, M., Buel, G. R., Niño, C. A., Maspero, E., Scotto di Perrotolo, R., Raimondi, A., ... & Polo, S. (2019). Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension. Nature communications, 10(1), 1-15.

Anderson, R. H., Kerkvliet, J. G., Otta, J. J., Ross, A. D., Leiferman, P. C., Hoppe, A. D., & Francis, K. R. (2018). Generation of a CLTA reporter human induced pluripotent stem cell line, CRMi001-A-1, using the CRISPR/Cas9 system to monitor endogenous clathrin trafficking. Stem cell research, 33, 95-99.

Lu, Y., Wang, J., Guo, X., Yan, S., & Dai, J. (2017). Perfluorooctanoic acid affects endocytosis involving clathrin light chain A and microRNA-133b-3p in mouse testes. Toxicology and Applied Pharmacology, 318, 41-48.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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Second antibody and isotype control

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