Mouse Anti-CLTA Recombinant Antibody (CBYY-C3188) (CBMAB-C4631-YY)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Rat

Clone

CBYY-C3188

Antibody Isotype

IgG1

Application

FC, IHC

Basic Information

Immunogen

PHA stimulated rat lymphocytes

Specificity

Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Protein G purified

Buffer

1 mg/mL

Preservative

PBS, pH 7.4

Concentration

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

clathrin, light chain (Lca)

Entrez Gene ID

83800

UniProt ID

P08081

Alternative Names

AHUS5, ARMD9, ASPa, C3b, CPAMD1, HEL-S-62p, C3

Function

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:21297582).

Biological Process

Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Clathrin coat assembly Source: CAFA
Clathrin-dependent endocytosis Source: GO_Central
Intracellular protein transport Source: InterPro
Low-density lipoprotein particle clearance Source: Reactome
Low-density lipoprotein particle receptor catabolic process Source: Reactome
Membrane organization Source: Reactome
Post-Golgi vesicle-mediated transport Source: Reactome

Cellular Location

Spindle; Cytoplasmic vesicle membrane; Coated pit. Cytoplasmic face of coated pits and vesicles. In complex with TACC3 and CKAP5 (forming the TACC3/ch-TOG/clathrin complex) localized to inter-microtubule bridges in mitotic spindles.

More Infomation

References

Redlingshöfer, L., McLeod, F., Chen, Y., Camus, M. D., Burden, J. J., Palomer, E., ... & Brodsky, F. M. (2020). Clathrin light chain diversity regulates membrane deformation in vitro and synaptic vesicle formation in vivo. Proceedings of the National Academy of Sciences, 117(38), 23527-23538.

Tsygankova, O. M., & Keen, J. H. (2019). A unique role for clathrin light chain A in cell spreading and migration. Journal of cell science, 132(10), jcs224030.

Biancospino, M., Buel, G. R., Niño, C. A., Maspero, E., Scotto di Perrotolo, R., Raimondi, A., ... & Polo, S. (2019). Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension. Nature communications, 10(1), 1-15.

Anderson, R. H., Kerkvliet, J. G., Otta, J. J., Ross, A. D., Leiferman, P. C., Hoppe, A. D., & Francis, K. R. (2018). Generation of a CLTA reporter human induced pluripotent stem cell line, CRMi001-A-1, using the CRISPR/Cas9 system to monitor endogenous clathrin trafficking. Stem cell research, 33, 95-99.

Lu, Y., Wang, J., Guo, X., Yan, S., & Dai, J. (2017). Perfluorooctanoic acid affects endocytosis involving clathrin light chain A and microRNA-133b-3p in mouse testes. Toxicology and Applied Pharmacology, 318, 41-48.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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Second antibody and isotype control

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