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Mouse Anti-CHAT Recombinant Antibody (CBLNC-155) (CBMAB-1354-CN)


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Published Data
CBMAB-1354-CN-1
Mouse Anti-CHAT Recombinant Antibody (CBLNC-155) (CBMAB-1354-CN) in IF
Immunofluorescence staining of NMDA1R (yellow), ChAT (green), and BDNF (magenta) in hippocampal primary culture. DAPI staining (blue) indicates the nucleus. Scale bar = 50 μm. ...View More
Lim, J., Bang, Y., Kim, K. M., & Choi, H. J. (2023). Differentiated HT22 cells as a novel model for in vitro screening of serotonin reuptake inhibitors. Frontiers in Pharmacology, 13, 1062650.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-1354-CN-2
Mouse Anti-CHAT Recombinant Antibody (CBLNC-155) (CBMAB-1354-CN) in WB
Western blotting (D) of HT22 cells grown in normal growth medium and differentiation medium for 3 d. ...View More
Lim, J., Bang, Y., Kim, K. M., & Choi, H. J. (2023). Differentiated HT22 cells as a novel model for in vitro screening of serotonin reuptake inhibitors. Frontiers in Pharmacology, 13, 1062650.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-1354-CN-3
Mouse Anti-CHAT Recombinant Antibody (CBLNC-155) (CBMAB-1354-CN) in IF
Immunofluorescence images of MN-like differentiated hEnSCs in different groups of TCP, collagen hydrogel without Mic EpoB (2-Col/PA-CH/M), collagen hydrogel with Mic EpoB (2-Col/PA-CH/Mic EpoB), and control (undifferentiated hEnSCs) for beta-III-tubulin, NES, ISL-1, CHAT, and HB9 after 15 days (× 20, Scale bar 100 μm). ...View More
Mahmoodi, N., Ai, J., Hassannejad, Z., Ebrahimi-Barough, S., Hasanzadeh, E., Nekounam, H., ... & Rahimi-Movaghar, V. (2021). Improving motor neuron-like cell differentiation of hEnSCs by the combination of epothilone B loaded PCL microspheres in optimized 3D collagen hydrogel. Scientific Reports, 11(1), 21722.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-1354-CN-4
Mouse Anti-CHAT Recombinant Antibody (CBLNC-155) (CBMAB-1354-CN) in IF
Double immunolabeling of the human oviduct tissue for CD34 (green, Alexa Fluor 488) and PGP 9.5 (red, Alexa Fluor 594)-(A); for CD34 and iNOS (red, Alexa Fluor 594)-(B); for CD34 and ChAT (red, Alexa Fluor 594)-(C). Nerves are presented as red filaments accompanied by green structures (telocytes and blood vessels) longitudinally. Total magnification: ×400. ...View More
Aleksandrovych, V., Wrona, A., Bereza, T., Pityński, K., & Gil, K. (2021). Oviductal telocytes in patients with uterine myoma. Biomedicines, 9(8), 1060.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-1354-CN-5
Mouse Anti-CHAT Recombinant Antibody (CBLNC-155) (CBMAB-1354-CN) in WB
Western blotting was conducted to confirm the expression of ChAT in the salivary glands. ...View More
Toan, N. K., Tai, N. C., Kim, S. A., & Ahn, S. G. (2021). Choline acetyltransferase induces the functional regeneration of the salivary gland in aging SAMP1/KL-/-mice. International Journal of Molecular Sciences, 22(1), 404.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBLNC-155
Antibody Isotype
IgG1, κ
Application
ELISA, IF, IP, WB, IHC-P

Basic Information

Immunogen
Choactase of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C.

Target

Full Name
ChAT
Introduction
Choline O-acetyltransferase (CHAT) catalyzes the biosynthesis of the neurotransmitter acetylcholine. CHAT is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Mutations in CHAT gene are associated with congenital myasthenic syndrome associated with episodic apnea, Alzheimer's disease and mild cognitive impairment.
Entrez Gene ID
Human1103
Monkey709977
Rat290567
Guinea pig100718930
UniProt ID
HumanP28329
MonkeyA0A2K5VTI9
RatP32738
Guinea pigH0V586
Alternative Names
CMS6; CMS1A; CMS1A2; CHOACTASE
Function
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Biological Process
Acetylcholine biosynthetic process Source: GO_Central
Neuromuscular synaptic transmission Source: GO_Central
Neurotransmitter transport Source: Reactome
Phosphatidylcholine biosynthetic process Source: Reactome
Cellular Location
Cytosol; Nucleus; Cytoplasm; Neuron projection; Synapse
Involvement in disease
Myasthenic syndrome, congenital, 6, presynaptic (CMS6): A form of congenital myasthenic syndrome, a group of disorders characterized by failure of neuromuscular transmission, including pre-synaptic, synaptic, and post-synaptic disorders that are not of autoimmune origin. Clinical features are easy fatigability and muscle weakness affecting the axial and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and leading to dysphonia). The symptoms fluctuate and worsen with physical effort. CMS6 affected individuals have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. CMS6 inheritance is autosomal recessive.
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For research use only. Not intended for any clinical use.

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