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Mouse Anti-CHAT Recombinant Antibody (CBFYC-1801) (CBMAB-C1863-FY)


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Published Data
CBMAB-C1863-FY-1
Mouse Anti-CHAT Recombinant Antibody (CBFYC-1801) (CBMAB-C1863-FY) in IHC
ChAT staining of lumbar spinal cord (left panel) (scale bar: 100 um). The mumber of motor neurons per ventral horn (right panel). ChAT-positive motor neutons, with cell body diameters of at least 20 um, were counted. ...View More
Yamamotoya, T., Hasei, S., Akasaka, Y., Ohata, Y., Nakatsu, Y., Kanna, M., ... & Asano, T. (2022). Involvement of neuronal and muscular Trk-fused gene (TFG) defects in the development of neurodegenerative diseases. Scientific reports, 12(1), 1966.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYC-1801
Antibody Isotype
IgG2b
Application
WB, IHC, IF

Basic Information

Immunogen
Recombinant protein corresponding to Human CHAT.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500
IHC-P1:500-1:1,000
IF(ICC)2-10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ChAT
Introduction
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.
Entrez Gene ID
290567
UniProt ID
A0A0G2JYQ6
Alternative Names
choline O-acetyltransferase; CHOACTase; choline acetylase; choline acetyltransferase; EC 2.3.1.6
Function
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Biological Process
Acetylcholine biosynthetic process Source: GO_Central
Neuromuscular synaptic transmission Source: GO_Central
Neurotransmitter transport Source: Reactome
Phosphatidylcholine biosynthetic process Source: Reactome
Cellular Location
Cytosol; Nucleus; Cytoplasm; Neuron projection; Synapse
Involvement in disease
Myasthenic syndrome, congenital, 6, presynaptic (CMS6): A form of congenital myasthenic syndrome, a group of disorders characterized by failure of neuromuscular transmission, including pre-synaptic, synaptic, and post-synaptic disorders that are not of autoimmune origin. Clinical features are easy fatigability and muscle weakness affecting the axial and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and leading to dysphonia). The symptoms fluctuate and worsen with physical effort. CMS6 affected individuals have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. CMS6 inheritance is autosomal recessive.
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For research use only. Not intended for any clinical use.

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