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Rat Anti-CD34 Recombinant Antibody (CBFYC-1222) (CBMAB-C1280-FY)


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Published Data
CBMAB-C1280-FY-1
Rat Anti-CD34 Recombinant Antibody (CBFYC-1222) (CBMAB-C1280-FY) in FC
Isolated and obtained an MSC population in which>90% of cells expressed the specific MSC markers CD90 and CD44 and 84.07% of the CD73 marker. Furthermore, after immunodepletion, the MSCs were negative for CD45 (0.9±0.5%). ...View More
Jiang, X., Xu, C., Shi, H., & Cheng, Q. (2019). PTH1-34 improves bone healing by promoting angiogenesis and facilitating MSCs migration and differentiation in a stabilized fracture mouse model. PLoS One, 14(12), e0226163. Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Rat
Specificity
Mouse
Clone
CBFYC-1222
Antibody Isotype
IgG2
Application
FC

Basic Information

Immunogen
Cell lysates of mouse endothelial cells.
Host Species
Rat
Specificity
Mouse
Antibody Isotype
IgG2
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
FC2.5 µg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CD34 Molecule
Introduction
CD34 (CD34 Molecule) is a Protein Coding gene. Diseases associated with CD34 include Dermatofibrosarcoma Protuberans and Gastrointestinal Stromal Tumor. Among its related pathways are Hematopoietic Stem Cell Differentiation Pathways and Lineage-specific Markers and Innate Immune System. Gene Ontology (GO) annotations related to this gene include transcription factor binding and sulfate binding.
Entrez Gene ID
12490
UniProt ID
Q543Y2
Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Biological Process
Cell-cell adhesion Source: HGNC-UCL
Cell-matrix adhesion Source: GO_Central
Cell motility Source: UniProtKB
Endothelial cell proliferation Source: UniProtKB
Endothelium development Source: UniProtKB
Extracellular exosome assembly Source: UniProtKB
Glomerular endothelium development Source: UniProtKB
Glomerular filtration Source: UniProtKB
Hematopoietic stem cell proliferation Source: UniProtKB
Hemopoiesis Source: UniProtKB
Leukocyte migration Source: HGNC-UCL
Mesangial cell-matrix adhesion Source: UniProtKB
Metanephric glomerular mesangial cell differentiation Source: UniProtKB
Negative regulation of blood coagulation Source: UniProtKB
Negative regulation of cellular response to heat Source: UniProtKB
Negative regulation of cellular response to hypoxia Source: UniProtKB
Negative regulation of gene expression Source: UniProtKB
Negative regulation of interleukin-2 production Source: UniProtKB
Negative regulation of neuron death Source: UniProtKB
Negative regulation of nitric oxide biosynthetic process Source: UniProtKB
Negative regulation of tumor necrosis factor production Source: UniProtKB
Paracrine signaling Source: UniProtKB
Positive regulation of angiogenesis Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of glial cell-derived neurotrophic factor production Source: UniProtKB
Positive regulation of granulocyte colony-stimulating factor production Source: UniProtKB
Positive regulation of interleukin-10 production Source: UniProtKB
Positive regulation of odontogenesis Source: UniProtKB
Positive regulation of transforming growth factor beta production Source: UniProtKB
Positive regulation of vasculogenesis Source: UniProtKB
Regulation of blood pressure Source: UniProtKB
Regulation of immune response Source: Reactome
Signal transduction Source: UniProtKB
Stem cell proliferation Source: UniProtKB
Tissue homeostasis Source: UniProtKB
Transdifferentiation Source: UniProtKB
Vascular wound healing Source: UniProtKB
Cellular Location
Membrane
Topology
Extracellular: 32-290
Helical: 291-311
Cytoplasmic: 312-385
PTM
Highly glycosylated.
Phosphorylated on serine residues by PKC.
More Infomation

Abs, D., Landman, S., Osio, A., Lepesant, P., Schneider, P., Obadia, D., ... & Battistella, M. (2021). Spindle cell tumor with CD34 and S100 co‐expression and distinctive stromal and perivascular hyalinization showing EML4‐ALK fusion. Journal of Cutaneous Pathology, 48(7), 896-901.

Michal, M., Ptáková, N., Martínek, P., Gatalica, Z., Kazakov, D. V., Michalová, K., ... & Michal, M. (2019). S100 and CD34 positive spindle cell tumor with prominent perivascular hyalinization and a novel NCOA4‐RET fusion. Genes, Chromosomes and Cancer, 58(9), 680-685.

Michaelsen, S. R., Urup, T., Olsen, L. R., Broholm, H., Lassen, U., & Poulsen, H. S. (2018). Molecular profiling of short-term and long-term surviving patients identifies CD34 mRNA level as prognostic for glioblastoma survival. Journal of Neuro-oncology, 137(3), 533-542.

Bellagamba, B. C., Grudzinski, P. B., Ely, P. B., Nader, P. D. J. H., Nardi, N. B., & da Silva Meirelles, L. (2018). Induction of expression of CD271 and CD34 in mesenchymal stromal cells cultured as spheroids. Stem cells international, 2018.

Sumide, K., Matsuoka, Y., Kawamura, H., Nakatsuka, R., Fujioka, T., Asano, H., ... & Sonoda, Y. (2018). A revised road map for the commitment of human cord blood CD34-negative hematopoietic stem cells. Nature communications, 9(1), 1-17.

Suurmeijer, A. J., Dickson, B. C., Swanson, D., Zhang, L., Sung, Y. S., Cotzia, P., ... & Antonescu, C. R. (2018). A novel group of spindle cell tumors defined by S100 and CD34 co‐expression shows recurrent fusions involving RAF1, BRAF, and NTRK1/2 genes. Genes, Chromosomes and Cancer, 57(12), 611-621.

Fan, J., Li, H., Nie, X., Yin, Z., Zhao, Y., Zhang, X., ... & Wang, D. W. (2018). MiR-665 aggravates heart failure via suppressing CD34-mediated coronary microvessel angiogenesis. Aging (Albany NY), 10(9), 2459.

Abe, T., Matsuoka, Y., Nagao, Y., Sonoda, Y., & Hanazono, Y. (2017). CD34-negative hematopoietic stem cells show distinct expression profiles of homing molecules that limit engraftment in mice and sheep. International journal of hematology, 106(5), 631-637.

Huse, J. T., Snuderl, M., Jones, D. T., Brathwaite, C. D., Altman, N., Lavi, E., ... & Rosenblum, M. K. (2017). Polymorphous low-grade neuroepithelial tumor of the young (PLNTY): an epileptogenic neoplasm with oligodendroglioma-like components, aberrant CD34 expression, and genetic alterations involving the MAP kinase pathway. Acta neuropathologica, 133(3), 417-429.

Foroozan, M., Roudi, R., Abolhasani, M., Gheytanchi, E., & Mehrazma, M. (2017). Clinical significance of endothelial cell marker CD34 and mast cell marker CD117 in prostate adenocarcinoma. Pathology-Research and Practice, 213(6), 612-618.

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For research use only. Not intended for any clinical use.

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