Rat Anti-CCR3 Recombinant Antibody (83103) (CBMAB-C8962-LY)

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Published Data

Rat Anti-CCR3 Recombinant Antibody (83103) (CBMAB-C8962-LY) in FC

Flow cytometry gating strategy of peritoneal macrophages.
Gating strategy for the identification by FACS analysis of neutrophils (CD45+, CD11c-,CD19-,CCR3-, CD11b+, LY6G+),eosinophils (CD45+, CD11c-,CD19-,CCR3-, CD11blow, LY6G-,F4/80low), monocytes (CD45+, CD11c-,CD19-,CCR3-, CD11b+,LY6G-,F4/80+, LY6C+), small peritoneal macrophages (SPM; CD45+, CD11c-,CD19-,CCR3-, CD11b+, LY6G-,F4/80low) andlarge peritoneal macrophages (LPM; CD45+, CD11c-, CD19-, CCR3-, CD11b+, LY6G-, F4/80hi). One representative example for peritoneal cells from an unstimulated animal is shown. ...View More

Pepe, G., Braga, D., Renzi, T. A., Villa, A., Bolego, C., D'Avila, F., ... & Vegeto, E. (2017). Self-renewal and phenotypic conversion are the main physiological responses of macrophages to the endogenous estrogen surge. Scientific reports, 7(1), 44270.

Datasheet

Summary

Host Animal

Rat

Specificity

Mouse

Clone

83103

Antibody Isotype

IgG2a

Application

Basic Information

Immunogen

Y3 rat myeloid cell line transfected with mouse CCR3 Met1-Phe359.

Host Species

Rat

Specificity

Mouse

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
FC	0.25-1 µg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, BSA

Preservative

< 0.1% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

C-C Motif Chemokine Receptor 3

Introduction

CCR3 (C-C Motif Chemokine Receptor 3) is a Protein Coding gene. Diseases associated with CCR3 include Folliculotropic Mycosis Fungoides and Aids Dementia Complex. Among its related pathways are Akt Signaling and IL1 and megakaryocytes in obesity. Gene Ontology (GO) annotations related to this gene include G-protein coupled receptor activity and C-C chemokine receptor activity.
An important paralog of this gene is CCR1.

Entrez Gene ID

12771

UniProt ID

P51678

Function

Receptor for C-C type chemokine. Binds and responds to a variety of chemokines, including CCL11, CCL26, CCL7, CCL13, RANTES(CCL5) and CCL15 (PubMed:7622448, PubMed:8642344, PubMed:8676064).
Subsequently transduces a signal by increasing the intracellular calcium ions level (PubMed:8676064).
In addition acts as a possible functional receptor for NARS1 (PubMed:30171954).
(Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection.

Biological Process

Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: ProtInc
Calcium-mediated signaling Source: GO_Central
Cell adhesion Source: ProtInc
Cell chemotaxis Source: GO_Central
Cellular defense response Source: ProtInc
Chemotaxis Source: ProtInc
G protein-coupled receptor signaling pathway Source: Reactome
Immune response Source: GO_Central
Inflammatory response Source: GO_Central
Positive regulation of angiogenesis Source: BHF-UCL
Positive regulation of cytosolic calcium ion concentration Source: GO_Central
Positive regulation of endothelial cell proliferation Source: Ensembl
Viral process Source: UniProtKB-KW

Cellular Location

Cell membrane

Topology

Extracellular: 1-34
Helical: 35-62
Cytoplasmic: 63-72
Helical: 73-93
Extracellular: 94-107
Helical: 108-129
Cytoplasmic: 130-146
Helical: 147-171
Extracellular: 172-203
Helical: 204-223
Cytoplasmic: 224-239
Helical: 240-264
Extracellular: 265-281
Helical: 282-305
Cytoplasmic: 306-355

More Infomation

References

van Aalst, E., Koneri, J., & Wylie, B. J. (2021). In Silico Identification of Cholesterol Binding Motifs in the Chemokine Receptor CCR3. Membranes, 11(8), 570.

Korbecki, J., Kojder, K., Simińska, D., Bohatyrewicz, R., Gutowska, I., Chlubek, D., & Baranowska-Bosiacka, I. (2020). CC chemokines in a tumor: a review of pro-cancer and anti-cancer properties of the ligands of receptors CCR1, CCR2, CCR3, and CCR4. International Journal of Molecular Sciences, 21(21), 8412.

Zhu, X., Liu, K., Wang, J., Peng, H., Pan, Q., Wu, S., ... & Liu, Y. (2018). C‑C chemokine receptor type 3 gene knockout alleviates inflammatory responses in allergic rhinitis model mice by regulating the expression of eosinophil granule proteins and immune factors. Molecular medicine reports, 18(4), 3780-3790.

Turner, J. D., Pionnier, N., Furlong-Silva, J., Sjoberg, H., Cross, S., Halliday, A., ... & Taylor, M. J. (2018). Interleukin-4 activated macrophages mediate immunity to filarial helminth infection by sustaining CCR3-dependent eosinophilia. PLoS pathogens, 14(3), e1006949.

Ge, B., Li, J., Wei, Z., Sun, T., Song, Y., & Khan, N. U. (2017). Functional expression of CCL8 and its interaction with chemokine receptor CCR3. BMC immunology, 18(1), 1-8.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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