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Rat Anti-CCR3 Recombinant Antibody (61828) (CBMAB-C7875-LY)


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Published Data
CBMAB-C7875-LY-1
Rat Anti-CCR3 Recombinant Antibody (61828) (CBMAB-C7875-LY) in Neutralization
hBMECs were starved overnight at 3 dpi, and ZIKV NS5 was quantified by Western blot analysis and normalized to GAPDH levels. ...View More
Mladinich, M. C., Conde, J. N., Schutt, W. R., Sohn, S. Y., & Mackow, E. R. (2021). Blockade of autocrine CCL5 responses inhibits Zika virus persistence and spread in human brain microvascular endothelial cells. MBio, 12(4), 10-1128.
CBMAB-C7875-LY-2
Rat Anti-CCR3 Recombinant Antibody (61828) (CBMAB-C7875-LY) in FC
Gating strategy used on neutrophils isolated from the BAL fluid and blood of ARDS patients. Following red blood cell lysis (30 sec in distilled H2O), neutrophils were incubated with the antibody cocktail described in the Methods section. FSC vs SSC was used to identify the leukocyte population and exclude thrombocytes and cell debris. SSC-A vs SSC-H and SSC-A vs SSC-W was used to exclude cell doublets. CD14+,
HLA-DR+ cells were then excluded to remove monocytes, macrophages and dendritic cells from the analysis. Neutrophils were finally gated as CD16+ (CD14-, HLA-DR-). The chemokine receptor expression (CXCR1, CXCR2, CCR1, CCR2 and CCR3) was then analysed on the neutrophil population. blood neutrophils. ...View More
Williams, A. E., José, R. J., Mercer, P. F., Brealey, D., Parekh, D., Thickett, D. R., ... & Chambers, R. C. (2017). Evidence for chemokine synergy during neutrophil migration in ARDS. Thorax, 72(1), 66-73.
CBMAB-C7875-LY-3
Rat Anti-CCR3 Recombinant Antibody (61828) (CBMAB-C7875-LY) in Neutralization
Human pulmonary microvascular endothelial cells (HPMEC) were seeded in 24-well plate pre-coated with matrigel (5 × 104/well). The total tube length was analyzed by ImageJ. CCL28 (2000 ng/ml) and VEGFA (10 ng/ml) significantly increased the formed tube length of HPMEC. In addition, the effect of CCL28 on tube formation was neutralized by antibody against CCR3 (CCR3 Ab). ...View More
Huang, G., Tao, L., Shen, S., & Chen, L. (2016). Hypoxia induced CCL28 promotes angiogenesis in lung adenocarcinoma by targeting CCR3 on endothelial cells. Scientific Reports, 6(1), 27152.

Summary

Host Animal
Rat
Specificity
Human
Clone
61828
Antibody Isotype
IgG2a
Application
FC, IHC, Neutralization

Basic Information

Immunogen
Y3 rat myeloid cell line transfected with human CCR3.
Host Species
Rat
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IHC8-25 µg/ml
FC2.5 µg/10^6 cells
Neutralization0.05-0.25 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
C-C Motif Chemokine Receptor 3
Introduction
CCR3 (C-C Motif Chemokine Receptor 3) is a Protein Coding gene. Diseases associated with CCR3 include Folliculotropic Mycosis Fungoides and Aids Dementia Complex. Among its related pathways are Akt Signaling and IL1 and megakaryocytes in obesity. Gene Ontology (GO) annotations related to this gene include G-protein coupled receptor activity and C-C chemokine receptor activity.
An important paralog of this gene is CCR1.
Entrez Gene ID
1232
UniProt ID
P51677
Alternative Names
C-C Motif Chemokine Receptor 3; Chemokine (C-C Motif) Receptor 3; Eosinophil Eotaxin Receptor; C-C CKR-3; CC-CKR-3; CMKBR3; CCR-3; CKR3;
Function
Receptor for C-C type chemokine. Binds and responds to a variety of chemokines, including CCL11, CCL26, CCL7, CCL13, RANTES(CCL5) and CCL15 (PubMed:7622448, PubMed:8642344, PubMed:8676064).
Subsequently transduces a signal by increasing the intracellular calcium ions level (PubMed:8676064).
In addition acts as a possible functional receptor for NARS1 (PubMed:30171954).
(Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection.
Biological Process
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: ProtInc
Calcium-mediated signaling Source: GO_Central
Cell adhesion Source: ProtInc
Cell chemotaxis Source: GO_Central
Cellular defense response Source: ProtInc
Chemotaxis Source: ProtInc
G protein-coupled receptor signaling pathway Source: Reactome
Immune response Source: GO_Central
Inflammatory response Source: GO_Central
Positive regulation of angiogenesis Source: BHF-UCL
Positive regulation of cytosolic calcium ion concentration Source: GO_Central
Positive regulation of endothelial cell proliferation Source: Ensembl
Viral process Source: UniProtKB-KW
Cellular Location
Cell membrane
Topology
Extracellular: 1-34
Helical: 35-62
Cytoplasmic: 63-72
Helical: 73-93
Extracellular: 94-107
Helical: 108-129
Cytoplasmic: 130-146
Helical: 147-171
Extracellular: 172-203
Helical: 204-223
Cytoplasmic: 224-239
Helical: 240-264
Extracellular: 265-281
Helical: 282-305
Cytoplasmic: 306-355
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For research use only. Not intended for any clinical use.

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