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Mouse Anti-CCNE1 Recombinant Antibody (HE12) (CBMAB-1327-CN)


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Published Data
CBMAB-1327-CN-1
Mouse Anti-CCNE1 Recombinant Antibody (HE12) (CBMAB-1327-CN) in IF
Co-localization of endogenous FANCD2 and transiently expressed GFP-tagged FBXL12 in U2OS CYCLIN E cells. Representative MLN4924-treated cell with intensity plots of FBXL12 and FANCD2 signals along the indicated blue line. Scale bars, 10 μm. Red, FANCD2; green, FBXL12; blue, Hoechst33342. ...View More
Brunner, A., Li, Q., Fisicaro, S., Kourtesakis, A., Viiliäinen, J., Johansson, H. J., ... & Sangfelt, O. (2023). FBXL12 degrades FANCD2 to regulate replication recovery and promote cancer cell survival under conditions of replication stress. Molecular Cell, 83(20), 3720-3739.
CBMAB-1327-CN-2
Mouse Anti-CCNE1 Recombinant Antibody (HE12) (CBMAB-1327-CN) in WB
Western blot was used to examine the protein expression of G2/M checkpoint regulators in UC cells. ...View More
Su, Y. L., Xiao, L. Y., Huang, S. Y., Wu, C. C., Chang, L. C., Chen, Y. H., ... & Peng, J. M. (2023). Inhibiting WEE1 augments the antitumor efficacy of Cisplatin in urothelial carcinoma by enhancing the DNA damage process. Cells, 12(11), 1471.
CBMAB-1327-CN-3
Mouse Anti-CCNE1 Recombinant Antibody (HE12) (CBMAB-1327-CN) in WB
Cell cycle-related markers were analyzed by using Western blotting according to MSO concentrations. ...View More
Lee, E., Seo, H. D., Kim, D., Park, S. H., Kim, S. R., Hyun, C., ... & Jung, C. H. (2023). Millet seed oil activates β-catenin signaling and promotes hair growth. Frontiers in Pharmacology, 14, 1172084.
CBMAB-1327-CN-4
Mouse Anti-CCNE1 Recombinant Antibody (HE12) (CBMAB-1327-CN) in WB
Western blot analysis of key genes involved in cell-cycle regulation in PC3 cells that silenced with LSM3, DHX16 or NOVA2. ...View More
Zhang, H., Tian, J., Ren, S., Han, B., Tian, R., Zuo, X., ... & Niu, R. (2023). Comprehensively analysis of splicing factors to construct prognosis prediction classifier in prostate cancer. Journal of Cellular and Molecular Medicine, 27(18), 2684-2700.
CBMAB-1327-CN-5
Mouse Anti-CCNE1 Recombinant Antibody (HE12) (CBMAB-1327-CN) in WB
Western blotting analysis of U251MG and U87MG. β-Actin was used as a loading control. C DNA content analysis using PI staining followed by flow cytometry. A.U.: arbitrary unit. ...View More
Kamikawa, Y., Wu, Z., Nakazawa, N., Ito, T., Saito, A., & Imaizumi, K. (2023). Impact of cell cycle on repair of ruptured nuclear envelope and sensitivity to nuclear envelope stress in glioblastoma. Cell Death Discovery, 9(1), 233.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
HE12
Antibody Isotype
IgG1, κ
Application
IF, IHC-P, WB, IP

Basic Information

Immunogen
Recombinant cyclin E of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C. Do not freeze.

Target

Full Name
Cyclin E1
Introduction
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.
Entrez Gene ID
Human898
Mouse12447
UniProt ID
HumanP24864
MouseQ61457
Alternative Names
CCNE; pCCNE1
Function
Essential for the control of the cell cycle at the G1/S (start) transition.
Biological Process
Cell division Source: UniProtKB-KW
DNA replication initiation Source: Ensembl
G1/S transition of mitotic cell cycle Source: BHF-UCL
Homologous chromosome pairing at meiosis Source: Ensembl
Mitotic cell cycle phase transition Source: GO_Central
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of mesenchymal stem cell proliferation Source: BHF-UCL
Protein phosphorylation Source: MGI
Regulation of cellular protein localization Source: Ensembl
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
Regulation of transcription involved in G1/S transition of mitotic cell cycle Source: Reactome
Telomere maintenance Source: Ensembl
Wnt signaling pathway Source: Ensembl
Cellular Location
Nucleus
PTM
Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7.
Ubiquitinated by UHRF2; appears to occur independently of phosphorylation.
More Infomation

Sonntag, R., Penners, C., Kohlhepp, M., Haas, U., Lambertz, D., Kroh, A., ... & Liedtke, C. (2021). Cyclin E1 in Murine and Human Liver Cancer: A Promising Target for Therapeutic Intervention during Tumour Progression. Cancers, 13(22), 5680.

Pascual, J., Gil-Gil, M., Zielinski, C., Hills, M., Ruiz-Borrego, M., Ciruelos, E. M., ... & Martin, M. (2021). CCNE1 mRNA and cyclin E1 protein expression as predictive biomarkers for efficacy of palbociclib plus fulvestrant versus capecitabine in the phase III PEARL study.

Zhu, W., Si, Y., Xu, J., Lin, Y., Wang, J. Z., Cao, M., ... & Wei, J. F. (2020). Methyltransferase like 3 promotes colorectal cancer proliferation by stabilizing CCNE1 mRNA in an m6A‐dependent manner. Journal of cellular and molecular medicine, 24(6), 3521-3533.

Turner, N. C., Liu, Y., Zhu, Z., Loi, S., Colleoni, M., Loibl, S., ... & Cristofanilli, M. (2019). Cyclin E1 expression and palbociclib efficacy in previously treated hormone receptor–positive metastatic breast cancer. Journal of Clinical Oncology, 37(14), 1169.

Turner, N. C., Liu, Y., Zhu, Z., Loi, S., Colleoni, M., Loibl, S., ... & Cristofanilli, M. (2018). Abstract CT039: Cyclin E1 (CCNE1) expression associates with benefit from palbociclib in metastatic breast cancer (MBC) in the PALOMA3 trial.

Sonntag, R., Giebeler, N., Nevzorova, Y. A., Bangen, J. M., Fahrenkamp, D., Lambertz, D., ... & Liedtke, C. (2018). Cyclin E1 and cyclin-dependent kinase 2 are critical for initiation, but not for progression of hepatocellular carcinoma. Proceedings of the National Academy of Sciences, 115(37), 9282-9287.

Huang, X., & Lyu, J. (2018). Tumor suppressor function of miR‑483‑3p on breast cancer via targeting of the cyclin E1 gene. Experimental and therapeutic medicine, 16(3), 2615-2620.

Ehedego, H., Mohs, A., Jansen, B., Hiththetiya, K., Sicinski, P., Liedtke, C., & Trautwein, C. (2018). Loss of Cyclin E1 attenuates hepatitis and hepatocarcinogenesis in a mouse model of chronic liver injury. Oncogene, 37(25), 3329-3339.

Guarducci, C., Bonechi, M., Benelli, M., Biagioni, C., Boccalini, G., Romagnoli, D., ... & Migliaccio, I. (2018). Cyclin E1 and Rb modulation as common events at time of resistance to palbociclib in hormone receptor-positive breast cancer. NPJ Breast Cancer, 4(1), 1-10.

Bangen, J. M., Hammerich, L., Sonntag, R., Baues, M., Haas, U., Lambertz, D., ... & Liedtke, C. (2017). Targeting CCl4‐induced liver fibrosis by RNA interference–mediated inhibition of cyclin E1 in mice. Hepatology, 66(4), 1242-1257.

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For research use only. Not intended for any clinical use.

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