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Mouse Anti-CCNE1 Recombinant Antibody (CBFYC-1044) (CBMAB-C1101-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYC-1044
Antibody Isotype
IgG1
Application
IHC-P

Basic Information

Immunogen
Recombinant Human CCNE1 / Cyclin-E1 protein.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IHC-P1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cyclin E1
Introduction
Cyclin E1 (CCNE1) gene amplification has been reported to occur independently of BRCA1/2 mutation, and it is associated with primary treatment failure and reduced patient survival.
Entrez Gene ID
898
UniProt ID
P24864
Alternative Names
Cyclin E1; CCNE; G1/S-Specific Cyclin-E1; Cyclin Es; Cyclin Et; PCCNE1
Function
Essential for the control of the cell cycle at the G1/S (start) transition.
Biological Process
Cell division Source: UniProtKB-KW
DNA replication initiation Source: Ensembl
G1/S transition of mitotic cell cycle Source: BHF-UCL
Homologous chromosome pairing at meiosis Source: Ensembl
Mitotic cell cycle phase transition Source: GO_Central
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of mesenchymal stem cell proliferation Source: BHF-UCL
Protein phosphorylation Source: MGI
Regulation of cellular protein localization Source: Ensembl
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
Regulation of transcription involved in G1/S transition of mitotic cell cycle Source: Reactome
Telomere maintenance Source: Ensembl
Wnt signaling pathway Source: Ensembl
Cellular Location
Nucleus
PTM
Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7.
Ubiquitinated by UHRF2; appears to occur independently of phosphorylation.
More Infomation

Sonntag, R., Penners, C., Kohlhepp, M., Haas, U., Lambertz, D., Kroh, A., ... & Liedtke, C. (2021). Cyclin E1 in Murine and Human Liver Cancer: A Promising Target for Therapeutic Intervention during Tumour Progression. Cancers, 13(22), 5680.

Pascual, J., Gil-Gil, M., Zielinski, C., Hills, M., Ruiz-Borrego, M., Ciruelos, E. M., ... & Martin, M. (2021). CCNE1 mRNA and cyclin E1 protein expression as predictive biomarkers for efficacy of palbociclib plus fulvestrant versus capecitabine in the phase III PEARL study.

Zhu, W., Si, Y., Xu, J., Lin, Y., Wang, J. Z., Cao, M., ... & Wei, J. F. (2020). Methyltransferase like 3 promotes colorectal cancer proliferation by stabilizing CCNE1 mRNA in an m6A‐dependent manner. Journal of cellular and molecular medicine, 24(6), 3521-3533.

Turner, N. C., Liu, Y., Zhu, Z., Loi, S., Colleoni, M., Loibl, S., ... & Cristofanilli, M. (2019). Cyclin E1 expression and palbociclib efficacy in previously treated hormone receptor–positive metastatic breast cancer. Journal of Clinical Oncology, 37(14), 1169.

Turner, N. C., Liu, Y., Zhu, Z., Loi, S., Colleoni, M., Loibl, S., ... & Cristofanilli, M. (2018). Abstract CT039: Cyclin E1 (CCNE1) expression associates with benefit from palbociclib in metastatic breast cancer (MBC) in the PALOMA3 trial.

Sonntag, R., Giebeler, N., Nevzorova, Y. A., Bangen, J. M., Fahrenkamp, D., Lambertz, D., ... & Liedtke, C. (2018). Cyclin E1 and cyclin-dependent kinase 2 are critical for initiation, but not for progression of hepatocellular carcinoma. Proceedings of the National Academy of Sciences, 115(37), 9282-9287.

Huang, X., & Lyu, J. (2018). Tumor suppressor function of miR‑483‑3p on breast cancer via targeting of the cyclin E1 gene. Experimental and therapeutic medicine, 16(3), 2615-2620.

Ehedego, H., Mohs, A., Jansen, B., Hiththetiya, K., Sicinski, P., Liedtke, C., & Trautwein, C. (2018). Loss of Cyclin E1 attenuates hepatitis and hepatocarcinogenesis in a mouse model of chronic liver injury. Oncogene, 37(25), 3329-3339.

Guarducci, C., Bonechi, M., Benelli, M., Biagioni, C., Boccalini, G., Romagnoli, D., ... & Migliaccio, I. (2018). Cyclin E1 and Rb modulation as common events at time of resistance to palbociclib in hormone receptor-positive breast cancer. NPJ Breast Cancer, 4(1), 1-10.

Bangen, J. M., Hammerich, L., Sonntag, R., Baues, M., Haas, U., Lambertz, D., ... & Liedtke, C. (2017). Targeting CCl4‐induced liver fibrosis by RNA interference–mediated inhibition of cyclin E1 in mice. Hepatology, 66(4), 1242-1257.

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For research use only. Not intended for any clinical use.

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