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Mouse Anti-ADIPOQ Recombinant Antibody (V2-6089) (CBMAB-0034CQ)


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Published Data
Mouse Anti-ADIPOQ Recombinant Antibody (19F1) (CBMAB-0034CQ) in WB
WB analysis of serum adiponectin levels in Adipoq-Cre or control (WT) mice injected with AAV2/8-FLEX-Becn1F121A and then fed with HFD for 12 weeks. n = 4. t test. ...View More
Kuramoto, K., Kim, Y. J., Hong, J. H., & He, C. (2021). The autophagy protein Becn1 improves insulin sensitivity by promoting adiponectin secretion via exocyst binding. Cell reports, 35(8), 109184.
Mouse Anti-ADIPOQ Recombinant Antibody (19F1) (CBMAB-0034CQ) in WB
Effect of whey peptides on 3T3-L1 adipocyte differentiation. 3T3-L1 preadipocytes were differentiated in the presence of 2.5 mg/mL WPI, WPH, BPI or BPH. Immunoblotting and densitometric analysis of (A,B) PPARγ, (A,C) C/EBPα, and (A,D) adiponectin (n = 6). mRNA levels of PPARγ (E) and PPARγ target genes, (F) adiponectin and (G) stearoyl-CoA desaturase (Scd1) (n = 8) were determined. TG levels (H) were measured using a targeted lipidomics approach in adipocytes incubated with BSA protein isolate or whey peptides (n = 8). (B-H): * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. protein isolate controls. BPI, BSA protein isolate; BPH, BSA peptide hydrolysate; WPI, whey protein isolate; WPH, whey peptide hydrolysate; PPARγ, peroxisome proliferator-activated receptor γ; C/EBPα, CCAAT/enhancer-binding protein α; PS, protein stain; Scd1, stearoyl-Coenzyme A desaturase 1; A.U., arbitrary units. ...View More
D'Souza, K., Mercer, A., Mawhinney, H., Pulinilkunnil, T., Udenigwe, C. C., & Kienesberger, P. C. (2020). Whey peptides stimulate differentiation and lipid metabolism in adipocytes and ameliorate lipotoxicity-induced insulin resistance in muscle cells. Nutrients, 12(2), 425.
Mouse Anti-ADIPOQ Recombinant Antibody (19F1) (CBMAB-0034CQ) in WB
Western blot analysis for total lung expression of adiponectin (26 kDa), AdipoR1 (43 kDa), and AdipoR2 (44 kDa) normalized to β-actin in the newborn rat during alveolarization (postnatal days (PD) 4, 10 and 21). APN is adiponectin, AdR1 and AdR2 are adiponectin receptor 1 and 2, respectively. ...View More
Kang, N. Y., Ivanovska, J., Tamir-Hostovsky, L., Belik, J., & Gauda, E. B. (2018). Chronic intermittent hypoxia in premature infants: The link between low fat stores, adiponectin receptor signaling and lung injury. In Arterial Chemoreceptors: New Directions and Translational Perspectives (pp. 151-157). Springer International Publishing.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Rabbit, Baboon
Clone
V2-6089
Antibody Isotype
IgG
Application
ELISA, ICC, IF, IHC-P, WB

Basic Information

Immunogen
Recombinant Human full length protein
Host Species
Mouse
Specificity
Human, Mouse, Rat, Rabbit, Baboon
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:100-1:2,000
FC1:10-1:1,000
IF(ICC)2-4 μg/ml
IHC1:20-1:100
IHC-P1:10-1:500
WB1 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, 1 mg/ml BSA
Preservative
0.05% sodium azide
Concentration
1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Adiponectin, C1Q And Collagen Domain Containing
Introduction
This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Adiponectin is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adipocytes also produce and secrete proteins such as leptin, adipsin, various other complement components, and tumor necrosis factor, suggesting a possible link to the immune system. Adiponectin, an adipose tissue-specific plasma protein, has antiinflammatory effects on the cellular components of the vascular wall Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Entrez Gene ID
Human9370
Mouse11450
Rat246253
Rabbit100009027
Baboon101014121
UniProt ID
HumanQ15848
MouseQ60994
RatQ8K3R4
RabbitG1TV17
BaboonA0A2I3LW74
Alternative Names
Adiponectin, C1Q And Collagen Domain Containing; Adipose Most Abundant Gene Transcript 1 Protein; Adipocyte Complement-Related 30 KDa Protein; 30 KDa Adipocyte Complement-Related Protein; ACRP30; APM-1; GBP28; ACDC; APM1; Adipocyte, C1q And Collagen Domain-Containing Protein; Adipocyte, C1Q And Collagen Domain Containing; Adipose Most Abundant Gene Transcript 1; Adipose Specific Collagen-Like Factor; Gelatin-Binding Protein 28; Gelatin-Binding Protein; Adiponectin Precursor; ADIPQTL1; ADPN
Function
Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism. Required for normal glucose and fat homeostasis and for maintaining a normal body weight. ADIPOQ-binding activates a signaling cascade that leads to increased AMPK activity, and ultimately to increased fatty acid oxidation, increased glucose uptake and decreased gluconeogenesis. Has high affinity for globular adiponectin and low affinity for full-length adiponectin (By similarity).
Biological Process
Adiponectin-activated signaling pathway
Fatty acid oxidation
Glucose homeostasis
Hormone-mediated signaling pathway
Leptin-mediated signaling pathway
Negative regulation of cell growth
Negative regulation of epithelial cell migration
Negative regulation of epithelial to mesenchymal transition
Negative regulation of NIK/NF-kappaB signaling
Negative regulation of receptor signaling pathway via JAK-STAT
Positive regulation of cold-induced thermogenesis
Positive regulation of insulin receptor signaling pathway
Positive regulation of receptor signaling pathway via JAK-STAT
Regulation of fatty acid biosynthetic process
Regulation of glucose metabolic process
Regulation of lipid metabolic process
Cellular Location
Cell membrane. Localized to the cell membrane and intracellular organelles.
Topology
Cytoplasmic: 1-136 aa
Helical: 137-157 aa
Extracellular: 158-170 aa
Helical: 171-191 aa
Cytoplasmic: 192-203 aa
Helical: 204-224 aa
Extracellular: 225-234 aa
Helical: 235-255 aa
Cytoplasmic: 256-264 aa
Helical: 265-285 aa
Extracellular: 286-298 aa
Helical: 299-319 aa
Cytoplasmic: 320-337 aa
Helical: 338-358 aa
Extracellular: 359-375 aa
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For research use only. Not intended for any clinical use.

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