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Mouse Anti-ADIPOQ Recombinant Antibody (V2-634147) (CBMAB-AP166LY)


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Published Data
Mouse Anti-ADIPOQ Recombinant Antibody (1G12) (CBMAB-AP166LY) in Immunoblot
Adiponectin increases AMPK activity and phosphorylation of ACC in the liver in vivo. a-f, Immunoblot analyses of phosphorylation of αAMPK (a and b) and ACC (e and f), and activity of α1AMPK (c) and α2AMPK (d), in the liver from mice treated with the indicated concentrations (µg per 10 g body weight) of gAd, full-length Ad, or AICAR (0.5 mM or 1 g/kg body weight). Measurements were made at the following times: in a, c and d, 5 min; in b, 2 min or 5 min; in f, 5 min or 15 min; and in e, 15 min. Each bar represents mean ± s.e. (n = 8-10). *, P < 0.05; **, P < 0.01; compared with vehicle. ...View More
Yamauchi, T., Kamon, J., Minokoshi, Y. A., Ito, Y., Waki, H., Uchida, S., ... & Kadowaki, T. (2002). Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase. Nature medicine, 8(11), 1288-1295.

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
V2-634147
Antibody Isotype
IgG1, κ
Application
Dot blot, ELISA, WB

Basic Information

Immunogen
Recombinant human adiponectin (15-244aa) purified from E. coli
Host Species
Mouse
Specificity
Human, Mouse
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH7.4
Preservative
0.01% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Adiponectin, C1Q And Collagen Domain Containing
Introduction
This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010]
Entrez Gene ID
Human9370
Mouse11450
UniProt ID
HumanQ15848
MouseQ60994
Alternative Names
Adiponectin, C1Q And Collagen Domain Containing; Adipose Most Abundant Gene Transcript 1 Protein; Adipocyte Complement-Related 30 KDa Protein; 30 KDa Adipocyte Complement-Related Protein; ACRP30; APM-1; GBP28; ACDC; APM1; Adipocyte, C1q And Collagen Domain-Containing Protein;
Function
Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism. Required for normal glucose and fat homeostasis and for maintaining a normal body weight. ADIPOQ-binding activates a signaling cascade that leads to increased AMPK activity, and ultimately to increased fatty acid oxidation, increased glucose uptake and decreased gluconeogenesis. Has high affinity for globular adiponectin and low affinity for full-length adiponectin (By similarity).
Biological Process
Adiponectin-activated signaling pathway
Fatty acid oxidation
Glucose homeostasis
Hormone-mediated signaling pathway
Leptin-mediated signaling pathway
Negative regulation of cell growth
Negative regulation of epithelial cell migration
Negative regulation of epithelial to mesenchymal transition
Negative regulation of NIK/NF-kappaB signaling
Negative regulation of receptor signaling pathway via JAK-STAT
Positive regulation of cold-induced thermogenesis
Positive regulation of insulin receptor signaling pathway
Positive regulation of receptor signaling pathway via JAK-STAT
Regulation of fatty acid biosynthetic process
Regulation of glucose metabolic process
Regulation of lipid metabolic process
Cellular Location
Cell membrane. Localized to the cell membrane and intracellular organelles.
Topology
Cytoplasmic: 1-136 aa
Helical: 137-157 aa
Extracellular: 158-170 aa
Helical: 171-191 aa
Cytoplasmic: 192-203 aa
Helical: 204-224 aa
Extracellular: 225-234 aa
Helical: 235-255 aa
Cytoplasmic: 256-264 aa
Helical: 265-285 aa
Extracellular: 286-298 aa
Helical: 299-319 aa
Cytoplasmic: 320-337 aa
Helical: 338-358 aa
Extracellular: 359-375 aa
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For research use only. Not intended for any clinical use.

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