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Mouse Anti-ACAN Recombinant Antibody (V2-179199) (CBMAB-A0456-YC)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-179199
Antibody Isotype
IgM
Application
FC, IHC, WB, ELISA, IP

Basic Information

Immunogen
Human articular cartilage aggrecan
Specificity
Human
Antibody Isotype
IgM
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:10,000
IF(ICC)1:200-1:1,000
WB1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Ascites
Preservative
0.03% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Aggrecan
Introduction
ACAN belongs to the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia
Entrez Gene ID
176
UniProt ID
P16112
Alternative Names
Aggrecan; Chondroitin Sulfate Proteoglycan Core Protein 1; Cartilage-Specific Proteoglycan Core Protein; Chondroitin Sulfate Proteoglycan 1; CSPG1; MSK16; AGC1; Large Aggregating Proteoglycan; Aggrecan Proteoglycan;
Function
This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
Biological Process
Cell adhesion
Central nervous system development
Extracellular matrix organization
Keratan sulfate biosynthetic process
Keratan sulfate catabolic process
Proteolysis
Skeletal system development
Cellular Location
Extracellular matrix
Involvement in disease
Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy.
A bone disease characterized by severe short stature, macrocephaly, severe midface hypoplasia, short neck, barrel chest and brachydactyly. The radiological findings comprise long bones with generalized irregular epiphyses with widened metaphyses, especially at the knees, platyspondyly, and multiple cervical-vertebral clefts.
An autosomal dominant disease characterized by short stature, advanced bone maturation, early-onset osteoarthritis, and mild dysmorphic features consisting of midface hypoplasia, brachydactyly, broad great toes, and lumbar lordosis. Other features include intervertebral disk disease and osteochondritis dissecans. Osteochondritis dissecans is defined as a separation of cartilage and subchondral bone from the surrounding tissue.
PTM
Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase.
More Infomation

König, K. C., Lahm, H., Dreßen, M., Doppler, S. A., Eichhorn, S., Beck, N., ... & Krane, M. (2021). Aggrecan: a new biomarker for acute type A aortic dissection. Scientific Reports, 11(1), 1-12.

Schmidt, S., Stapf, C., Schmutzler, S., Lachmann, I., Arendt, T., Holzer, M., ... & Morawski, M. (2020). Aggrecan modulates the expression and phosphorylation of tau in a novel bigenic TauP301L‐Acan mouse model. European Journal of Neuroscience.

Tashkandi, M. M., Alsaqer, S. F., Alhousami, T., Ali, F., Wu, Y. C., Shin, J., ... & Bais, M. V. (2020). LOXL2 promotes aggrecan and gender-specific anabolic differences to TMJ cartilage. Scientific reports, 10(1), 1-13.

de Andrade, D. G. A., Basso, R. M., Magro, A. J., Laufer-Amorim, R., Borges, A. S., & de Oliveira-Filho, J. P. (2020). Evaluation of a new variant in the aggrecan gene potentially associated with chondrodysplastic dwarfism in Miniature horses. Scientific reports, 10(1), 1-10.

Dupuis, L. E., Nelson, E. L., Hozik, B., Porto, S. C., Rogers-DeCotes, A., Fosang, A., & Kern, C. B. (2019). Adamts5−/− mice exhibit altered aggrecan proteolytic profiles that correlate with ascending aortic anomalies. Arteriosclerosis, thrombosis, and vascular biology, 39(10), 2067-2081.

Struck, A. K., Dierks, C., Braun, M., Hellige, M., Wagner, A., Oelmaier, B., ... & Distl, O. (2018). A recessive lethal chondrodysplasia in a miniature zebu family results from an insertion affecting the chondroitin sulfat domain of aggrecan. BMC genetics, 19(1), 1-9.

Zhang, B., Guo, W., Sun, C., Duan, H. Q., Yu, B. B., Mu, K., ... & Feng, S. Q. (2018). Dysregulated MiR-3150a-3p promotes lumbar intervertebral disc degeneration by targeting aggrecan. Cellular Physiology and Biochemistry, 45(6), 2506-2515.

Sentchordi‐Montané, L., Aza‐Carmona, M., Benito‐Sanz, S., Barreda‐Bonis, A. C., Sánchez‐Garre, C., Prieto‐Matos, P., ... & Heath, K. E. (2018). Heterozygous aggrecan variants are associated with short stature and brachydactyly: description of 16 probands and a review of the literature. Clinical endocrinology, 88(6), 820-829.

Rowlands, D., Lensjø, K. K., Dinh, T., Yang, S., Andrews, M. R., Hafting, T., ... & Dick, G. (2018). Aggrecan directs extracellular matrix-mediated neuronal plasticity. Journal of Neuroscience, 38(47), 10102-10113.

Gkourogianni, A., Andrew, M., Tyzinski, L., Crocker, M., Douglas, J., Dunbar, N., ... & Dauber, A. (2017). Clinical characterization of patients with autosomal dominant short stature due to aggrecan mutations. The Journal of Clinical Endocrinology & Metabolism, 102(2), 460-469.

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For research use only. Not intended for any clinical use.

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