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Mouse Anti-ACAN Monoclonal Antibody (V2-5181) (CBMAB-0929-YC)


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Published Data
Mouse Anti-ACAN Monoclonal Antibody (7D4) (CBMAB-0929-YC) in IHC
Distribution of extracellular matrix (ECM) in the superior (SVN) vestibular nuclei using biotinylated hyaluronan-binding protein to detect aggrecan (M-P). ...View More
Gaál, B., Rácz, É., Juhász, T., Holló, K., & Matesz, C. (2014). Distribution of extracellular matrix macromolecules in the vestibular nuclei and cerebellum of the frog, Rana esculenta. Neuroscience, 258, 162-173.
Mouse Anti-ACAN Monoclonal Antibody (7D4) (CBMAB-0929-YC) in IHC
Light micrographs of sections through bovine explants that had been cultured for 6 weeks under the indicated stimulation conditions and then either stained with Toluidine Blue (1st row), or treated for the immunohistochemical detection of aggrecan. ...View More
Shintani, N., Siebenrock, K. A., & Hunziker, E. B. (2013). TGF-ss1 enhances the BMP-2-induced chondrogenesis of bovine synovial explants and arrests downstream differentiation at an early stage of hypertrophy. PloS one, 8(1), e53086.

Summary

Host Animal
Mouse
Specificity
Human, Cattle
Clone
V2-5181
Antibody Isotype
IgG1
Application
ELISA, IHC-Fr, IP, IHC-P, WB

Basic Information

Immunogen
Purified human articular cartilage aggrecan
Specificity
Human, Cattle
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.09% sodium azide
Concentration
1 mg/ml
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Aggrecan
Introduction
Aggrecan is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage, which is a member of the aggrecan/versican proteoglycan family. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration.
Entrez Gene ID
Human176
Cattle280985
UniProt ID
HumanP16112
CattleP13608
Alternative Names
AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP; SSOAOD
Function
This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
Biological Process
Cell adhesion
Central nervous system development
Extracellular matrix organization
Keratan sulfate biosynthetic process
Keratan sulfate catabolic process
Proteolysis
Skeletal system development
Cellular Location
Extracellular matrix
Involvement in disease
Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy.
A bone disease characterized by severe short stature, macrocephaly, severe midface hypoplasia, short neck, barrel chest and brachydactyly. The radiological findings comprise long bones with generalized irregular epiphyses with widened metaphyses, especially at the knees, platyspondyly, and multiple cervical-vertebral clefts.
An autosomal dominant disease characterized by short stature, advanced bone maturation, early-onset osteoarthritis, and mild dysmorphic features consisting of midface hypoplasia, brachydactyly, broad great toes, and lumbar lordosis. Other features include intervertebral disk disease and osteochondritis dissecans. Osteochondritis dissecans is defined as a separation of cartilage and subchondral bone from the surrounding tissue.
PTM
Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase.
More Infomation

König, K. C., Lahm, H., Dreßen, M., Doppler, S. A., Eichhorn, S., Beck, N., ... & Krane, M. (2021). Aggrecan: a new biomarker for acute type A aortic dissection. Scientific Reports, 11(1), 1-12.

Schmidt, S., Stapf, C., Schmutzler, S., Lachmann, I., Arendt, T., Holzer, M., ... & Morawski, M. (2020). Aggrecan modulates the expression and phosphorylation of tau in a novel bigenic TauP301L‐Acan mouse model. European Journal of Neuroscience.

Tashkandi, M. M., Alsaqer, S. F., Alhousami, T., Ali, F., Wu, Y. C., Shin, J., ... & Bais, M. V. (2020). LOXL2 promotes aggrecan and gender-specific anabolic differences to TMJ cartilage. Scientific reports, 10(1), 1-13.

de Andrade, D. G. A., Basso, R. M., Magro, A. J., Laufer-Amorim, R., Borges, A. S., & de Oliveira-Filho, J. P. (2020). Evaluation of a new variant in the aggrecan gene potentially associated with chondrodysplastic dwarfism in Miniature horses. Scientific reports, 10(1), 1-10.

Dupuis, L. E., Nelson, E. L., Hozik, B., Porto, S. C., Rogers-DeCotes, A., Fosang, A., & Kern, C. B. (2019). Adamts5−/− mice exhibit altered aggrecan proteolytic profiles that correlate with ascending aortic anomalies. Arteriosclerosis, thrombosis, and vascular biology, 39(10), 2067-2081.

Struck, A. K., Dierks, C., Braun, M., Hellige, M., Wagner, A., Oelmaier, B., ... & Distl, O. (2018). A recessive lethal chondrodysplasia in a miniature zebu family results from an insertion affecting the chondroitin sulfat domain of aggrecan. BMC genetics, 19(1), 1-9.

Zhang, B., Guo, W., Sun, C., Duan, H. Q., Yu, B. B., Mu, K., ... & Feng, S. Q. (2018). Dysregulated MiR-3150a-3p promotes lumbar intervertebral disc degeneration by targeting aggrecan. Cellular Physiology and Biochemistry, 45(6), 2506-2515.

Sentchordi‐Montané, L., Aza‐Carmona, M., Benito‐Sanz, S., Barreda‐Bonis, A. C., Sánchez‐Garre, C., Prieto‐Matos, P., ... & Heath, K. E. (2018). Heterozygous aggrecan variants are associated with short stature and brachydactyly: description of 16 probands and a review of the literature. Clinical endocrinology, 88(6), 820-829.

Rowlands, D., Lensjø, K. K., Dinh, T., Yang, S., Andrews, M. R., Hafting, T., ... & Dick, G. (2018). Aggrecan directs extracellular matrix-mediated neuronal plasticity. Journal of Neuroscience, 38(47), 10102-10113.

Gkourogianni, A., Andrew, M., Tyzinski, L., Crocker, M., Douglas, J., Dunbar, N., ... & Dauber, A. (2017). Clinical characterization of patients with autosomal dominant short stature due to aggrecan mutations. The Journal of Clinical Endocrinology & Metabolism, 102(2), 460-469.

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For research use only. Not intended for any clinical use.

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