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HSPA5

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. This protein localizes to the lumen of the endoplasmic reticulum (ER) where it operates as a typical HSP70 chaperone involved in the folding and assembly of proteins in the ER and is a master regulator of ER homeostasis. During cellular stress, as during viral infection or tumorogenesis, this protein interacts with the transmembrane stress sensor proteins PERK (protein kinase R-like endoplasmic reticulum kinase), IRE1 (inositol-requiring kinase 1), and ATF6 (activating transcription factor 6) where it acts as a repressor of the unfolded protein response (UPR) and also plays a role in cellular apoptosis and senescence. Elevated expression and atypical translocation of this protein to the cell surface has been reported in viral infections and some types of cancer cells. At the cell surface this protein may facilitate viral attachment and entry to host cells. This gene is a therapeutic target for the treatment of coronavirus diseases and chemoresistant cancers.
Full Name
Heat Shock Protein Family A (Hsp70) Member 5
Alternative Names
BIP; GRP78; HEL-S-89n
Function
Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (PubMed:2294010, PubMed:23769672, PubMed:23990668, PubMed:28332555).

Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (By similarity).

Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (PubMed:1550958, PubMed:19538957).

In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity).

Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity).

Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating. May also play a role in apoptosis and cell proliferation (PubMed:26045166).

(Microbial infection) Plays an important role in viral binding to the host cell membrane and entry for several flaviruses such as Dengue virus, Zika virus and Japanese encephalitis virus (PubMed:33432092, PubMed:15098107, PubMed:28053106).

Acts as a component of the cellular receptor for Dengue virus serotype 2/DENV-2 on human liver cells (PubMed:15098107).

(Microbial infection) Acts as a receptor for CotH proteins expressed by fungi of the order mucorales, the causative agent of mucormycosis, which plays an important role in epithelial cell invasion by the fungi (PubMed:24355926, PubMed:20484814, PubMed:32487760).

Acts as a receptor for R.delemar CotH3 in nasal epithelial cells, which may be an early step in rhinoorbital/cerebral mucormycosis (RCM) disease progression (PubMed:32487760).
Biological Process
Cellular response to antibiotic Source: Ensembl
Cellular response to calcium ion Source: Ensembl
Cellular response to cAMP Source: Ensembl
Cellular response to gamma radiation Source: Ensembl
Cellular response to glucose starvation Source: UniProtKB
Cellular response to interleukin-4 Source: Ensembl
Cellular response to manganese ion Source: Ensembl
Cellular response to nerve growth factor stimulus Source: Ensembl
Cellular response to unfolded protein Source: GO_Central
Cellular response to xenobiotic stimulus Source: Ensembl
Cerebellar Purkinje cell layer development Source: Ensembl
Cerebellum structural organization Source: Ensembl
Chaperone cofactor-dependent protein refolding Source: GO_Central
Endoplasmic reticulum unfolded protein response Source: GO_Central
ER overload response Source: Ensembl
Luteolysis Source: Ensembl
Maintenance of protein localization in endoplasmic reticulum Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of IRE1-mediated unfolded protein response Source: UniProtKB
Negative regulation of protein-containing complex assembly Source: UniProtKB
Negative regulation of transforming growth factor beta receptor signaling pathway Source: Ensembl
Neuron apoptotic process Source: Ensembl
Neuron differentiation Source: Ensembl
Positive regulation of cell migration Source: UniProtKB
Positive regulation of neuron projection development Source: Ensembl
Positive regulation of protein ubiquitination Source: Ensembl
Positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
Posttranslational protein targeting to membrane, translocation Source: UniProtKB
Protein folding in endoplasmic reticulum Source: ParkinsonsUK-UCL
Protein refolding Source: GO_Central
Regulation of ATF6-mediated unfolded protein response Source: ParkinsonsUK-UCL
Regulation of IRE1-mediated unfolded protein response Source: ParkinsonsUK-UCL
Regulation of PERK-mediated unfolded protein response Source: ParkinsonsUK-UCL
Regulation of protein folding in endoplasmic reticulum Source: BHF-UCL
Response to cocaine Source: Ensembl
Response to methamphetamine hydrochloride Source: Ensembl
Stress response to metal ion Source: Ensembl
Substantia nigra development Source: UniProtKB
Toxin transport Source: Ensembl
Ubiquitin-dependent ERAD pathway Source: GO_Central
Cellular Location
Endoplasmic reticulum lumen; Cytoplasm; Melanosome; Cell surface. Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545). Localizes to the cell surface of epithelial cells in response to high levels of free iron (PubMed:20484814, PubMed:24355926, PubMed:27159390).
Involvement in disease
Autoantigen in rheumatoid arthritis.
PTM
AMPylated by FICD (PubMed:25601083). In unstressed cells, AMPylation at Thr-518 by FICD inactivates the chaperome activity: AMPylated form is locked in a relatively inert state and only weakly stimulated by J domain-containing proteins (By similarity). In response to endoplasmic reticulum stress, de-AMPylation by the same protein, FICD, restores the chaperone activity (By similarity).

Anti-HSPA5 antibodies

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Target: HSPA5
Host: Mouse
Antibody Isotype: IgG
Specificity: Human, Rat
Clone: EC174
Application*: WB, P
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG
Specificity: Human, Rat
Clone: EC173
Application*: WB, P
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG
Specificity: Human, Mouse
Clone: A301
Application*: IF, WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG
Specificity: Human, Mouse, Rat
Clone: A300
Application*: ELISA, ICC, WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG
Specificity: Human, Rat
Clone: CAP900
Application*: IHC, WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG
Specificity: Human, Rat
Clone: 5F8
Application*: IHC, WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG
Specificity: Human, Mouse, Rat
Clone: CAP899
Application*: ICC, WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Mouse, Rat
Clone: CB85A
Application*: ELISA, WB, ICC
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG
Specificity: Human, Rat
Clone: CB254
Application*: WB, IHC
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human
Clone: 4E11
Application*: WB, E
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 3B4-H11-C9
Application*: WB, IH
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG
Specificity: Human, Rat
Clone: CBLG1-2302
Application*: WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBLG1-2300
Application*: E (IA)
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human
Clone: CBLG1-2299
Application*: E (IA)
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Mouse, Rat
Clone: CBLG1-2298
Application*: E (IA)
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Rat
Clone: CBLG1-2297
Application*: IC/IF, P, WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Mouse
Clone: C9-9
Application*: WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: AT3D2
Application*: WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG
Specificity: Human, Rat
Clone: 8G3
Application*: WB
Target: HSPA5
Host: Rat
Antibody Isotype: IgG1
Specificity: Mouse, Human
Clone: 76-E6
Application*: WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Mouse
Clone: 4E3
Application*: IH, WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 42773
Application*: WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Rat
Clone: 13k16
Application*: IC, WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG
Specificity: Human, Rat
Clone: 10C9
Application*: WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human
Clone: C38
Application*: E, F
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Mouse, Rat, Rabbit, Cattle, Monkey, Hamster, Frog
Clone: 6H4-2G7
Application*: E, WB
Target: HSPA5
Host: Rat
Antibody Isotype: IgG1, κ
Specificity: Mouse
Clone: 5K23
Application*: E (IA), WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG
Specificity: Cattle, Cat, Chicken, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Frog
Clone: 4E88
Application*: IC, IP, WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Rat, Yeast
Clone: 4E87
Application*: E, F
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human
Clone: 474421
Application*: WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: 3D2
Application*: WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Rat, Human, Mouse
Clone: 3C5-1A4
Application*: E, WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG
Specificity: Human, Rat
Clone: 15B165
Application*: IC, WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Rat, Human
Clone: 10C3
Application*: F
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Rat
Clone: EG1453
Application*: WB, ICC
Target: HSPA5
Host: Rat
Antibody Isotype: IgG1, κ
Specificity: Mouse
Clone: CBFYH-3373
Application*: E, WB
Target: HSPA5
Host: Rat
Antibody Isotype: IgG1
Specificity: Mouse
Clone: CBFYH-3593
Application*: E, FN, WB
Target: HSPA5
Host: Human
Specificity: Mouse, Human
Clone: CBFYH-2282
Application*: WB
Target: HSPA5
Host: Rat
Antibody Isotype: IgG1, κ
Specificity: Mouse
Clone: CBFYH-2281
Application*: E, FN, WB
Target: HSPA5
Host: Rat
Antibody Isotype: IgG2a
Specificity: Mouse
Clone: CBFYH-2278
Application*: E, WB
Target: HSPA5
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human, Mouse
Clone: CBFYH-2277
Application*: E, WB, P, C
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Rat
Clone: CBFYH-2276
Application*: WB, IF
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Rat
Clone: CBFYH-2272
Application*: E, IC, IH, IP, WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human
Clone: CBFYH-2271
Application*: E, WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human, Mouse, Rat
Clone: CBFYH-2270
Application*: WB, E
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBFYH-2267
Application*: E, P, WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBFYH-2265
Application*: E, IH, WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG
Specificity: Human, Rat, Monkey
Clone: CBFYH-3240
Application*: WB
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human, Mouse, Rat
Clone: CBFYH-3239
Application*: WB, IF, E
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: CBFYH-2269
Application*: E
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human, Mouse, Rat
Clone: CBFYH-3238
Application*: WB, IF
Target: HSPA5
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 6H4.2G7
Application*: E, WB
More Infomation
For Research Use Only. Not For Clinical Use.
(P): Predicted
* Abbreviations
IFImmunofluorescence
IHImmunohistochemistry
IPImmunoprecipitation
WBWestern Blot
EELISA
MMicroarray
CIChromatin Immunoprecipitation
FFlow Cytometry
FNFunction Assay
IDImmunodiffusion
RRadioimmunoassay
TCTissue Culture
GSGel Supershift
NNeutralization
BBlocking
AActivation
IInhibition
DDepletion
ESELISpot
DBDot Blot
MCMass Cytometry/CyTOF
CTCytotoxicity
SStimulation
AGAgonist
APApoptosis
IMImmunomicroscopy
BABioassay
CSCostimulation
EMElectron Microscopy
IEImmunoelectrophoresis
PAPeptide Array
ICImmunocytochemistry
PEPeptide ELISA
MDMeDIP
SHIn situ hybridization
IAEnzyme Immunoassay
SEsandwich ELISA
PLProximity Ligation Assay
ECELISA(Cap)
EDELISA(Det)
BIBioimaging
IOImmunoassay
LFLateral Flow Immunoassay
LALuminex Assay
CImmunohistochemistry-Frozen Sections
PImmunohistologyp-Paraffin Sections
ISIntracellular Staining for Flow Cytometry
MSElectrophoretic Mobility Shift Assay
RIRNA Binding Protein Immunoprecipitation (RIP)
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