GRK1

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq]

Full Name

G protein-coupled receptor kinase 1

Function

Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade (PubMed:15946941).

This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination (By similarity).

May play a role in the maintenance of the outer nuclear layer in the retina (By similarity).

Biological Process

Protein autophosphorylation Source: UniProtKB
Protein phosphorylation Source: GO_Central
Regulation of G protein-coupled receptor signaling pathway Source: ProtInc
Regulation of rhodopsin mediated signaling pathway Source: UniProtKB
Regulation of signal transduction Source: GO_Central
Rhodopsin mediated signaling pathway Source: ProtInc
Visual perception Source: ProtInc

Cellular Location

Membrane; Photoreceptor outer segment. Subcellular location is not affected by light or dark conditions.

Involvement in disease

Night blindness, congenital stationary, Oguchi type 2 (CSNBO2):
A non-progressive retinal disorder characterized by impaired night vision, often associated with nystagmus and myopia. Congenital stationary night blindness Oguchi type is associated with fundus discoloration and abnormally slow dark adaptation.

PTM

Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
Farnesylation is required for full activity.

Anti-GRK1 antibodies

Mouse Anti-GRK1 Monoclonal Antibody (G8) (CBMAB-1339-YC)

Datasheet

SDS

Target: GRK1

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human, Mouse, Rat, Chicken, Cattle, Dog

Clone: G8

Application*: IC, IF, C, WB

Mouse Anti-GRK1 Monoclonal Antibody (D11) (CBMAB-1338-YC)

Datasheet

SDS

Target: GRK1

Host: Mouse

Antibody Isotype: IgG

Specificity: Human, Mouse, Cattle

Clone: D11

Application*: IC, IF, C, IP, WB

Mouse Anti-GRK1 Recombinant Antibody (8H207) (CBMAB-R0538-CN)

Datasheet

SDS

Target: GRK1

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human, Mouse, Rat

Clone: 8H207

Application*: WB

Mouse Anti-GRK1 Recombinant Antibody (CBLG1-1780) (CBMAB-G4985-LY)

Datasheet

SDS

Target: GRK1

Host: Mouse

Antibody Isotype: IgG2b, κ

Specificity: Human

Clone: CBLG1-1780

Application*: E, EC, WB

Mouse Anti-GRK1 Recombinant Antibody (CBLG1-1779) (CBMAB-G4984-LY)

Datasheet

SDS

Target: GRK1

Host: Mouse

Antibody Isotype: IgG

Specificity: Human

Clone: CBLG1-1779

Application*: IF, IP, WB

Mouse Anti-GRK1 Recombinant Antibody (CBLG1-1778) (CBMAB-G4983-LY)

Datasheet

SDS

Target: GRK1

Host: Mouse

Antibody Isotype: IgG2b

Specificity: Human

Clone: CBLG1-1778

Application*: WB

Mouse Anti-GRK1 Recombinant Antibody (CBLG1-1777) (CBMAB-G4982-LY)

Datasheet

SDS

Target: GRK1

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: CBLG1-1777

Application*: WB

Mouse Anti-GRK1 Recombinant Antibody (CBLG1-1776) (CBMAB-G4981-LY)

Datasheet

SDS

Target: GRK1

Host: Mouse

Antibody Isotype: IgG2b, κ

Specificity: Human

Clone: CBLG1-1776

Application*: E, WB

Mouse Anti-GRK1 Recombinant Antibody (CBLG1-1775) (CBMAB-G4980-LY)

Datasheet

SDS

Target: GRK1

Host: Mouse

Antibody Isotype: IgG2b, κ

Specificity: Human

Clone: CBLG1-1775

Application*: SE, E, WB

Mouse Anti-GRK1 Recombinant Antibody (1G8) (CBMAB-G4977-LY)

Datasheet

SDS

Target: GRK1

Host: Mouse

Antibody Isotype: IgG2b, κ

Specificity: Human

Clone: 1G8

Application*: E, WB

Mouse Anti-GRK1 Recombinant Antibody (466630) (CBMAB-G0796-LY)

Datasheet

SDS

Target: GRK1

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human, Mouse, Rat

Clone: 466630

Application*: WB

Mouse Anti-GRK1 Recombinant Antibody (CBFYH-0484) (CBMAB-H1354-FY)

Datasheet

SDS

Target: GRK1

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human, Mouse, Rat, Cattle, Chicken

Clone: CBFYH-0484

Application*: WB, IF

Mouse Anti-GRK1 Recombinant Antibody (CBFYH-0483) (CBMAB-H0101-FY)

Datasheet

SDS

Target: GRK1

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human, Cattle

Clone: CBFYH-0483

Application*: WB, IH, IF, C

More Infomation

For Research Use Only. Not For Clinical Use.

(P): Predicted

* Abbreviations

IFImmunofluorescence

IHImmunohistochemistry

IPImmunoprecipitation

WBWestern Blot

EELISA

MMicroarray

CIChromatin Immunoprecipitation

FFlow Cytometry

FNFunction Assay

IDImmunodiffusion

RRadioimmunoassay

TCTissue Culture

GSGel Supershift

NNeutralization

BBlocking

AActivation

IInhibition

DDepletion

ESELISpot

DBDot Blot

MCMass Cytometry/CyTOF

CTCytotoxicity

SStimulation

AGAgonist

APApoptosis

IMImmunomicroscopy

BABioassay

CSCostimulation

EMElectron Microscopy

IEImmunoelectrophoresis

PAPeptide Array

ICImmunocytochemistry

PEPeptide ELISA

MDMeDIP

SHIn situ hybridization

IAEnzyme Immunoassay

SEsandwich ELISA

PLProximity Ligation Assay

ECELISA(Cap)

EDELISA(Det)

BIBioimaging

IOImmunoassay

LFLateral Flow Immunoassay

LALuminex Assay

CImmunohistochemistry-Frozen Sections

PImmunohistologyp-Paraffin Sections

ISIntracellular Staining for Flow Cytometry

MSElectrophoretic Mobility Shift Assay

RIRNA Binding Protein Immunoprecipitation (RIP)