Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-GRK1 Recombinant Antibody (466630) (CBMAB-G0796-LY)


See all GRK1 antibodies
Online Inquiry

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
466630
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
E. coli-derived recombinant human GRK1, Leu343-Ser563, Accession # Q15835
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
lyophilized
Buffer
Trehalose
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
G protein-coupled receptor kinase 1
Introduction
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human6011
Mouse24013
Rat81760
UniProt ID
HumanQ15835
MouseQ9WVL4
RatQ63651
Alternative Names
G Protein-Coupled Receptor Kinase 1; Rhodopsin Kinase; EC 2.7.11.14; RHOK; RK; EC 2.7.11; GPRK1;
Function
Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade (PubMed:15946941).

This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination (By similarity).

May play a role in the maintenance of the outer nuclear layer in the retina (By similarity).
Biological Process
Protein autophosphorylation Source: UniProtKB
Protein phosphorylation Source: GO_Central
Regulation of G protein-coupled receptor signaling pathway Source: ProtInc
Regulation of rhodopsin mediated signaling pathway Source: UniProtKB
Regulation of signal transduction Source: GO_Central
Rhodopsin mediated signaling pathway Source: ProtInc
Visual perception Source: ProtInc
Cellular Location
Membrane; Photoreceptor outer segment. Subcellular location is not affected by light or dark conditions.
Involvement in disease
Night blindness, congenital stationary, Oguchi type 2 (CSNBO2):
A non-progressive retinal disorder characterized by impaired night vision, often associated with nystagmus and myopia. Congenital stationary night blindness Oguchi type is associated with fundus discoloration and abnormally slow dark adaptation.
PTM
Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
Farnesylation is required for full activity.
More Infomation

Millette, M. A., Roy, S., & Salesse, C. (2022). Farnesylation and lipid unsaturation are critical for the membrane binding of the C-terminal segment of G-Protein Receptor Kinase 1. Colloids and Surfaces B: Biointerfaces, 211, 112315.

Gulati, S., & Palczewski, K. (2022). Structural view of G protein-coupled receptor signaling in the retinal rod outer segment. Trends in Biochemical Sciences.

Chen, Q., Plasencia, M., Li, Z., Mukherjee, S., Patra, D., Chen, C. L., ... & Tesmer, J. J. (2021). Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1. Nature, 595(7868), 600-605.

Sulon, S. M., & Benovic, J. L. (2021). Targeting G protein–coupled receptor kinases to G protein–coupled receptors. Current opinion in endocrine and metabolic research, 16, 56-65.

Benovic, J. L. (2021). Historical perspective of the G protein-coupled receptor kinase family. Cells, 10(3), 555.

Kolesnikov, A. V., Chrispell, J. D., Osawa, S., Kefalov, V. J., & Weiss, E. R. (2020). Phosphorylation at Serine 21 in G protein-coupled receptor kinase 1 (GRK1) is required for normal kinetics of dark adaption in rod but not cone photoreceptors. FASEB journal: official publication of the Federation of American Societies for Experimental Biology, 34(2), 2677.

Yu, S., Sun, L., Jiao, Y., & Lee, L. T. O. (2018). The role of G protein-coupled receptor kinases in cancer. International journal of biological sciences, 14(2), 189.

Komolov, K. E., & Benovic, J. L. (2018). G protein-coupled receptor kinases: Past, present and future. Cellular signalling, 41, 17-24.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-GRK1 Recombinant Antibody (466630)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quoteOnline Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry