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Mouse Anti-AKR1C3 Recombinant Antibody (V2-180479) (CBMAB-A1993-YC)


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Published Data
Mouse Anti-AKR1C3 Recombinant Antibody (871701) in WB
Validation of stable knockdown of AKR1C3 in SKGT-4 cells and overexpression of AKR1C3 in OE33 cells. ...View More
Zhou, C., Wang, Z., Li, J., Wu, X., Fan, N., Li, D., ... & Zhao, Y. (2021). Aldo-keto reductase 1C3 mediates chemotherapy resistance in esophageal adenocarcinoma via ROS detoxification. Cancers, 13(10), 2403.
Mouse Anti-AKR1C3 Recombinant Antibody (871701) in IHC
Expression of androgen-metabolizing enzymes in the HFA at GW10 identified by immunohistochemistry. Inserts show the absence of immunostaining in the presence of non-immune serum instead of primary antibody. Scale bar: 100 μm. ...View More
Savchuk, I., Morvan, M. L., Antignac, J. P., Gemzell-Danielsson, K., Le Bizec, B., Söder, O., & Svechnikov, K. (2017). Androgenic potential of human fetal adrenals at the end of the first trimester. Endocrine connections, 6(6), 348-359.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-180479
Antibody Isotype
IgG1
Application
IF, IHC, WB

Basic Information

Immunogen
E. coli-derived recombinant human Aldo-keto Reductase 1C3/AKR1C3 Asp2-Tyr323.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB0.5 μg/ml
IF(ICC)8-25 μg/ml
IHC1-25 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Aldo-Keto Reductase Family 1 Member C3
Introduction
AKR1C3 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactor
Entrez Gene ID
Human8644
Pig733694
UniProt ID
HumanP42330
PigF1RYU4
Alternative Names
Aldo-Keto Reductase Family 1 Member C3; Prostaglandin F Synthase; Trans-1,2-Dihydrobenzene-1,2-Diol Dehydrogenase; 3-Alpha Hydroxysteroid Dehydrogenase, Type II; Testosterone 17-Beta-Dehydrogenase 5; Chlordecone Reductase Homolog HAKRb; Dihydrodiol Dehydr
Function
Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Acts as a NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain and regulates the metabolism of androgens, estrogens and progesterone (PubMed:10622721, PubMed:11165022, PubMed:7650035, PubMed:9415401, PubMed:9927279). Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH (PubMed:14672942, PubMed:11165022). Acts preferentially as a 17-ketosteroid reductase and has the highest catalytic efficiency of the AKR1C enzyme for the reduction of delta4-androstenedione to form testosterone (PubMed:20036328). Reduces prostaglandin (PG) D2 to 11beta-prostaglandin F2, progesterone to 20alpha-hydroxyprogesterone and estrone to 17beta-estradiol (PubMed:15047184, PubMed:20036328, PubMed:10622721, PubMed:11165022, PubMed:10998348, PubMed:19010934). Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol) (PubMed:10998348, PubMed:14672942, PubMed:11165022, PubMed:7650035, PubMed:9415401, PubMed:10557352). Displays also retinaldehyde reductase activity toward 9-cis-retinal (PubMed:21851338).
Biological Process
Cellular response to cadmium ion Source: UniProtKB
Cellular response to calcium ion Source: UniProtKB
Cellular response to corticosteroid stimulus Source: UniProtKB
Cellular response to jasmonic acid stimulus Source: UniProtKB
Cellular response to prostaglandin D stimulus Source: UniProtKB
Cellular response to prostaglandin stimulus Source: UniProtKB
Cellular response to reactive oxygen species Source: UniProtKB
Cellular response to starvation Source: UniProtKB
Cyclooxygenase pathway Source: Reactome
Daunorubicin metabolic process Source: UniProtKB
Doxorubicin metabolic process Source: UniProtKB
Farnesol catabolic process Source: UniProtKB
G protein-coupled receptor signaling pathway Source: UniProtKB
Keratinocyte differentiation Source: UniProtKB
Macromolecule metabolic process Source: UniProtKB
Male gonad development Source: UniProtKB
Negative regulation of retinoic acid biosynthetic process Source: UniProtKB
Positive regulation of cell death Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of endothelial cell apoptotic process Source: UniProtKB
Positive regulation of protein kinase B signaling Source: UniProtKB
Positive regulation of reactive oxygen species metabolic process Source: UniProtKB
Progesterone metabolic process Source: UniProtKB
Prostaglandin metabolic process Source: UniProtKB
Regulation of retinoic acid receptor signaling pathway Source: UniProtKB
Regulation of testosterone biosynthetic process Source: UniProtKB
Renal absorption Source: UniProtKB
Response to nutrient Source: UniProtKB
Retinal metabolic process Source: UniProtKB
Retinoid metabolic process Source: Reactome
Retinol metabolic process Source: GOC
Steroid metabolic process Source: UniProtKB
Testosterone biosynthetic process Source: UniProtKB
Cellular Location
Cytoplasm
More Infomation

Zhu, P., Feng, R., Lu, X., Liao, Y., Du, Z., Zhai, W., & Chen, K. (2021). Diagnostic and prognostic values of AKR1C3 and AKR1D1 in hepatocellular carcinoma. Aging (Albany NY), 13(3), 4138.

Zhou, C., Wang, Z., Li, J., Wu, X., Fan, N., Li, D., ... & Zhao, Y. (2021). Aldo-Keto Reductase 1C3 Mediates Chemotherapy Resistance in Esophageal Adenocarcinoma via ROS Detoxification. Cancers, 13(10), 2403.

Arafah, K., Kriegsmann, M., Renner, M., Lasitschka, F., Fresnais, M., Kriegsmann, K., ... & Longuespée, R. (2020). Microproteomics and Immunohistochemistry Reveal Differences in Aldo‐Keto Reductase Family 1 Member C3 in Tissue Specimens of Ulcerative Colitis and Crohn's Disease. PROTEOMICS–Clinical Applications, 14(4), 1900110.

Hertzog, J. R., Zhang, Z., Bignan, G., Connolly, P. J., Heindl, J. E., Janetopoulos, C. J., ... & McDevitt, T. M. (2020). AKR1C3 mediates pan‐AR antagonist resistance in castration‐resistant prostate cancer. The Prostate, 80(14), 1223-1232.

Zhao, J., Zhang, M., Liu, J., Liu, Z., Shen, P., Nie, L., ... & Zeng, H. (2019). AKR1C3 expression in primary lesion rebiopsy at the time of metastatic castration‐resistant prostate cancer is strongly associated with poor efficacy of abiraterone as a first‐line therapy. The Prostate, 79(13), 1553-1562.

Miyazaki, Y., Teramoto, Y., Shibuya, S., Goto, T., Okasho, K., Mizuno, K., ... & Inoue, T. (2019). Consecutive Prostate Cancer Specimens Revealed Increased Aldo–Keto Reductase Family 1 Member C3 Expression with Progression to Castration-Resistant Prostate Cancer. Journal of clinical medicine, 8(5), 601.

Liu, J., He, P., Lin, L., Zhao, Y., Deng, W., Ding, H., ... & Wang, Z. (2019). Characterization of a highly specific monoclonal antibody against human aldo-keto reductase AKR1C3. Steroids, 143, 73-79.

Hashimoto, Y., Imai, A., Yamamoto, H., Hatakeyama, S., Yoneyama, T., & Ohyama, C. (2018). Aldo-keto-reductase 1C3 expression in prostate cancer. Annals of Oncology, 29, ix71-ix72.

Penning, T. M. (2017). Aldo-Keto Reductase (AKR) 1C3 inhibitors: a patent review. Expert opinion on therapeutic patents, 27(12), 1329-1340.

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For research use only. Not intended for any clinical use.

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