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Mouse Anti-AKR1C1 Recombinant Antibody (V2-31915) (CBMAB-D0005-YC)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
V2-31915
Antibody Isotype
IgG2a, κ
Application
WB, IF, IHC, FC

Basic Information

Immunogen
Recombinant protein within Human AKR1C1 aa 180-323.
Host Species
Mouse
Specificity
Human, Mouse
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:2,000-1:10,000
IF(ICC)1:50-1:200
IHC1:50-1:200
FC1:50-1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4, 50% Glycerol, 0.2% BSA
Preservative
0.05% sodium azide
Concentration
2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Aldo-Keto Reductase Family 1 Member C1
Introduction
AKR1C1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. AKR1C1 catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone.
Entrez Gene ID
Human1645
Pig733634
UniProt ID
HumanQ04828
PigG5DGE2
Alternative Names
Aldo-Keto Reductase Family 1 Member C1; Dihydrodiol Dehydrogenase 1; 20-Alpha (3-Alpha)-Hydroxysteroid Dehydrogenase; High-Affinity Hepatic Bile Acid-Binding Protein; Trans-1,2-Dihydrobenzene-1,2-Diol Dehydrogenase; Chlordecone Reductase Homolog HAKRC; Dihydrodiol Dehydrogenase 1/2; Indanol Dehydrogenase; 20-ALPHA-HSD; DD1/DD2; DDH1; HBAB; DDH; Aldo-Keto Reductase Family 1, Member C1 (Dihydrodiol Dehydrogenase 1; 20-Alpha (3-Alpha)-Hydroxysteroid Dehydrogenase); Type II 3-Alpha-Hydroxysteroid Dehydrogenase; 20 Alpha-Hydroxysteroid Dehydrogenase; Dihydrodiol Dehydrogenase Isoform DD1;
Function
Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids (PubMed:19218247). Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH (PubMed:14672942). Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens (PubMed:10998348). May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate (PubMed:19218247). Displays affinity for bile acids (PubMed:8486699).
Biological Process
Bile acid and bile salt transport Source: UniProtKB
Bile acid metabolic process Source: UniProtKB
Cellular response to jasmonic acid stimulus Source: UniProtKB
Cholesterol homeostasis Source: UniProtKB
Daunorubicin metabolic process Source: UniProtKB
Digestion Source: UniProtKB
Doxorubicin metabolic process Source: UniProtKB
Epithelial cell differentiation Source: UniProtKB
Intestinal cholesterol absorption Source: UniProtKB
Progesterone metabolic process Source: UniProtKB
Prostaglandin metabolic process Source: GO_Central
Response to organophosphorus Source: UniProtKB
Retinal metabolic process Source: UniProtKB
Retinoid metabolic process Source: Reactome
Steroid metabolic process Source: GO_Central
Xenobiotic metabolic process
Cellular Location
Cytosol
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For research use only. Not intended for any clinical use.

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