Cytoskeleton is an interconnected assembly of actin, intermediate filament and microtubule network that extends throughout the entire cell. The cytoskeleton is involved in intracellular signal transduction such as regulation of cell shape, motility, transport and interactions with the environment. The cytoskeleton may affect signaling indirectly, for example, by regulating intracellular vesicle trafficking. Alternatively, physical contact between the cytoskeleton and signaling factors could modulate the spatiotemporal signaling output.
Fig.1 Organizational structures and physical interactions of actin, microtubules, and intermediate filaments.1
Different mechanisms underlie the cytoskeleton-dependent regulation of cell signaling. Changing the properties of the cytoskeleton provides ample opportunities for the fine-tuning of signaling events. Creative Biolabs offers cytoskeleton signal pathway phosphorylation screening service with antibody array, which consists of 141 high-specific antibodies and more than 70 phosphorylation sites involved in cytoskeleton signal pathways can be tested.
| Signal Pathways | |
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| Targets |
| ACTIN a/b/g pan (Y55/Y53) | ERK8 (T175/Y177) | MEK1(S221) | PIP5K (S307) |
| Calmodulin (T79/S81) | Ezrin (T566) | MEK1(T291) | PKA CAT (T197) |
| CAMK1A (T177) | Ezrin (Y353) | MEK2 (T394) | PRKCA (Y657) |
| CAMK2 (T305) | Ezrin (Y478) | Merlin (S10) | PRKCA/PRKCB2 (T638) |
| CAMK2B/CAMK2G/CAMK2D (T287) | FAK (S910) | Merlin (S518) | PKC pan phospho-activation sites |
| CAMK4 (T196/T200) | FAK (Y397) | MKK3 (S189) | PLC-beta (S1105) |
| CAMK2 (T286) | FAK (Y407) | MKK3/MAP2K3 (T222) | PLCbeta3 (S537) |
| Cofilin (S3) | FAK (Y576) | MKK6 (S207) | Rac1/cdc42 (S71) |
| Cortactin (Y421) | FAK (Y861) | MKK7/MAP2K7 (S271) | ARHGEF2 (S885) |
| Cortactin (Y466) | FAK (Y925) | MKK7/MAP2K7 (T275) | Src (S75) |
| c-Raf (S296) | FLNA (S2152) | MYL2 (S18) | Src (Y216) |
| c-Raf (S43) | GRB2 (S159) | p130Cas (Y165) | Src (Y418) |
| CrkII (Y221) | GAP (S387) | p130Cas (Y410) | Src (Y529) |
| CrkL (Y207) | LIMK1 (T508) | Paxillin (Y118) | VASP (S157) |
| Erk1/2 (T202) | MEK1 (S298) | Paxillin (Y31) | VASP (S238) |
| Erk1/2 (Y204) | MEK1 (T286) | PIK3R1 (Y607) | WASP (Y290) |
| ERK3 (S189) | MEK1(S217) | PIK3R1/PIK3R3 (Y467/Y199) | WAVE1 (Y125) |
For more detailed information about our antibody arrays, please feel free to contact us.
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