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Human Bone Metabolism Antibody Array (Glass Slide, 31 Targets) (Y0400) (AbAr-0400-YC)


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Specifications

Target Array
Bone Metabolism Array
Target
Activin A, aFGF(FGF-1), Amphiregulin, bFGF, BMP-4, BMP-9, E-Selectin, ICAM-1(CD54), IGF-1, IL-1 alpha(IL-1 F1), IL-1 beta(IL-1 F2), IL-6, IL-8(CXCL8), IL-11, IL-17A, MCP-1(CCL2), M-CSF, MIP-1 alpha(CCL3), MMP-2, MMP-9, MMP-13, Osteoactivin(GPNMB), P-Cadherin, RANK(TNFRSF11A), SDF-1 alpha(CXCL12 alpha), Sonic Hedgehog N-Terminal(Shh-N), TGF beta 1, TGF beta 2, TNF alpha, VCAM-1(CD106), VE-Cadherin(CDH5)
Species Reactivity
Human
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based Assay
Protocol Outline
Block glass slide surface
Incubate with samples
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Fluor Conjugated Streptavidin
Scan with an antibody array laser scanner
Analyze data
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction
Bone metabolism is a continual cycle of bone growth and resorption that is carefully orchestrated by the dynamic relationship between osteoclasts, osteoblasts and an array of hormonal and regulatory influences. The relative levels of these signaling molecules dictate whether healthy, balanced bone metabolism ensues.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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