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Human Bone Metabolism Antibody Array (Glass Slide, 31 Targets) (Y0128) (AbAr-0128-YC)


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Specifications

Target Array
Bone Metabolism Array
Target
Activin A, BMP-9, IL-1 beta (IL-1 F2), MCP-1 (CCL2), MMP-13, Sonic Hedgehog N-Terminal (Shh-N), VE-Cadherin (CDH5), aFGF (FGF-1), E-Selectin, IL-6, M-CSF, Osteoactivin (GPNMB), TGF beta 1, Amphiregulin, ICAM-1 (CD54), IL-8 (CXCL8), MIP-1 alpha (CCL3), P-Cadherin, TGF beta 2, bFGF, IGF-1, IL-11, MMP-2, RANK (TNFRSF11A), TNF alpha, BMP-4, IL-1 alpha (IL-1 F1), IL-17A, MMP-9, SDF-1 alpha (CXCL12 alpha), VCAM-1 (CD106)
Species Reactivity
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates, Other Body Fluids

Applications

Application
Multiplex Protein Detection
Assay Type
Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based
Protocol Outline
Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
Bone metabolism is a continual cycle of bone growth and resorption that is carefully orchestrated by the dynamic relationship between osteoclasts, osteoblasts and an array of hormonal and regulatory influences. The relative levels of these signaling molecules dictate whether healthy, balanced bone metabolism ensues.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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