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Mouse Anti-VIM Recombinant Antibody (3E9) (CBMAB-A9882-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
3E9
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
VIM (AAH30573, 41 a.a. ~ 140 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Vimentin
Introduction
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract
Entrez Gene ID
7431
UniProt ID
P08670
Alternative Names
FLJ36605
Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Biological Process
Astrocyte developmentIEA:Ensembl
Bergmann glial cell differentiationIEA:Ensembl
Cellular response to interferon-gammaIEA:Ensembl
Cellular response to lipopolysaccharideManual Assertion Based On ExperimentIMP:UniProtKB
Cellular response to muramyl dipeptideManual Assertion Based On ExperimentIMP:UniProtKB
Intermediate filament organizationISS:UniProtKB
Lens fiber cell developmentIEA:Ensembl
Negative regulation of neuron projection developmentIEA:Ensembl
Positive regulation of collagen biosynthetic processManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of translationManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of mRNA stabilityManual Assertion Based On ExperimentIMP:UniProtKB
SMAD protein signal transductionIEA:Ensembl
Cellular Location
Cytoplasm
Cytoplasm, cytoskeleton
Nucleus matrix
Cell membrane
Involvement in disease
Cataract 30, multiple types (CTRCT30):
An opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function.
PTM
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity).
One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm (PubMed:21465480).
Phosphorylated by STK33 (PubMed:18811945).
Phosphorylated on tyrosine residues by SRMS (PubMed:29496907).
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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