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Mouse Anti-VIM Recombinant Antibody (40E-C) (CBMAB-0197-CN)


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Summary

Host Animal
Mouse
Specificity
Canary, Avian, Echinoderm, Human, Mouse, Rat
Clone
40E-C
Antibody Isotype
IgM
Application
IF, IHC

Basic Information

Immunogen
Brain (adult canary); homogenized.
Specificity
Canary, Avian, Echinoderm, Human, Mouse, Rat
Antibody Isotype
IgM
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Vimentin
Introduction
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.
Entrez Gene ID
Human7431
Mouse22352
Rat81818
UniProt ID
HumanP08670
MouseP20152
RatP31000
Alternative Names
VIM
Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Biological Process
Astrocyte developmentIEA:Ensembl
Bergmann glial cell differentiationIEA:Ensembl
Cellular response to interferon-gammaIEA:Ensembl
Cellular response to lipopolysaccharideManual Assertion Based On ExperimentIMP:UniProtKB
Cellular response to muramyl dipeptideManual Assertion Based On ExperimentIMP:UniProtKB
Intermediate filament organizationISS:UniProtKB
Lens fiber cell developmentIEA:Ensembl
Negative regulation of neuron projection developmentIEA:Ensembl
Positive regulation of collagen biosynthetic processManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of translationManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of mRNA stabilityManual Assertion Based On ExperimentIMP:UniProtKB
SMAD protein signal transductionIEA:Ensembl
Cellular Location
Cytoplasm
Cytoplasm, cytoskeleton
Nucleus matrix
Cell membrane
Involvement in disease
Cataract 30, multiple types (CTRCT30):
An opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function.
PTM
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity).
One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm (PubMed:21465480).
Phosphorylated by STK33 (PubMed:18811945).
Phosphorylated on tyrosine residues by SRMS (PubMed:29496907).
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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