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Mouse Anti-USP30 Recombinant Antibody (CBXU-093) (CBMAB-U0192-CQ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXU-093
Antibody Isotype
IgG2a
Application
WB, IHC-P

Basic Information

Immunogen
Recombinant protein corresponding to amino acid sequence: IEAKGTLNGEKVEHQRTTFVKQLKLGKLPQCLCIHLQRLSWSSHGTPLKRHEHVQFNEFLMMDIYKYHLLGHKPSQHNPKLNKNPGPTLELQDGPGAPTPVLNQPGAPKTQIFMNGACSPSLLPTLSAPMPFPLPVVPDYSSST
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95% as determined by SDS-PAGE analysis
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ubiquitin specific peptidase 30
Introduction
USP30, a member of the ubiquitin-specific protease family (see USP1, MIM 603478), is a novel mitochondrial deubiquitinating (DUB) enzyme (Nakamura and Hirose, 2008 [PubMed 18287522]).
Entrez Gene ID
84749
UniProt ID
Q70CQ3
Alternative Names
USP30; ubiquitin specific peptidase 30; ubiquitin specific protease 30; ubiquitin carboxyl-terminal hydrolase 30; FLJ40511; MGC10702; ub-specific protease 30; ubiquitin thioesterase 30; deubiquitinating enzyme 30
Function
Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy (PubMed:18287522, PubMed:24896179, PubMed:25527291, PubMed:25621951).
Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling (PubMed:25621951).
Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65' (PubMed:25527291).
Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2 (By similarity).
Biological Process
Biological Process autophagy of mitochondrion Source:UniProtKB1 Publication
Biological Process mitochondrial fusion Source:UniProtKB
Biological Process negative regulation of mitophagy Source:Ensembl
Biological Process pexophagy Source:Reactome
Biological Process protein deubiquitination Source:UniProtKB1 Publication
Biological Process protein K11-linked deubiquitination Source:UniProtKB1 Publication
Biological Process protein K6-linked deubiquitination Source:UniProtKB1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:InterPro
Cellular Location
Mitochondrion outer membrane
Topology
Mitochondrial intermembrane: 1-35
Helical: 36-56
Cytoplasmic: 57-517
PTM
Ubiquitinated by parkin (PRKN) at Lys-235 and Lys-289, leading to its degradation.
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For research use only. Not intended for any clinical use.

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