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Mouse Anti-PBK Monoclonal Antibody (3A11) (CBMAB-1439-YC)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
3A11
Antibody Isotype
IgG2a, κ
Application
ELISA, ICC, IF, IHC, IHC-P, WB

Basic Information

Immunogen
Partial recombinant protein PBK (AAH15191, aa 1-110) with GST tag
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
PDZ Binding Kinase
Introduction
PBK is a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. PBK may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of PBK has been implicated in tumorigenesis.
Entrez Gene ID
55827
UniProt ID
Q96KB5
Alternative Names
SPK; CT84; TOPK; HEL164; Nori-3
Function
Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Biological Process
Cellular response to UVIEA:Ensembl
Mitotic cell cycle1 PublicationNAS:UniProtKB
Negative regulation of inflammatory responseIEA:Ensembl
Negative regulation of proteasomal ubiquitin-dependent protein catabolic processIEA:Ensembl
Negative regulation of protein phosphorylationIEA:Ensembl
Negative regulation of stress-activated MAPK cascadeIEA:Ensembl
Cellular Location
Nucleus
PTM
Phosphorylated; in a cell-cycle dependent manner at mitosis.
More Infomation

Wu, H., Qian, J., Zhou, L., Hu, T., Zhang, Y., Wang, C., ... & Gu, C. (2024). FHND004 inhibits malignant proliferation of multiple myeloma by targeting PDZ-binding kinase in MAPK pathway. Aging (Albany NY), 16(5), 4811.

Mu, W., Xie, Y., Li, J., Yan, R., Zhang, J., Liu, Y. E., & Fan, Y. (2022). High expression of PDZ-binding kinase is correlated with poor prognosis and immune infiltrates in hepatocellular carcinoma. World Journal of Surgical Oncology, 20(1), 22.

Miki, Y., Devi, L., Imai, Y., Minami, N., Koide, T., & Goel, S. (2020). Deletion of the PDZ-binding kinase (Pbk) gene does not affect male fertility in mice. Reproduction, Fertility and Development, 32(10), 893-902.

Mao, P., Bao, G., Wang, Y. C., Du, C. W., Yu, X., Guo, X. Y., ... & Wang, M. D. (2020). PDZ-binding kinase-dependent transcriptional regulation of CCNB2 promotes tumorigenesis and radio-resistance in glioblastoma. Translational oncology, 13(2), 287-294.

Chen, D., Cox, J., Annam, J., Weingart, M., Essien, G., Rathi, K. S., ... & Schnepp, R. W. (2020). LIN28B promotes neuroblastoma metastasis and regulates PDZ binding kinase. Neoplasia, 22(6), 231-241.

Kar, A., Zhang, Y., Yacob, B. W., Saeed, J., Tompkins, K. D., Bagby, S. M., ... & Kiseljak-Vassiliades, K. (2019). Targeting PDZ-binding kinase is anti-tumorigenic in novel preclinical models of ACC. Endocrine-related cancer, 26(10), 765-778.

Warren, A. Y., Massie, C. E., Watt, K., Luko, K., Orafidiya, F., Selth, L. A., ... & Asim, M. (2019). A reciprocal feedback between the PDZ binding kinase and androgen receptor drives prostate cancer. Oncogene, 38(7), 1136-1150.

Zhang, Y., Yang, X., Wang, R., & Zhang, X. (2019). Prognostic value of PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK) in patients with cancer. Journal of Cancer, 10(1), 131.

Diao, X., Yang, D., Chen, Y., & Liu, W. (2019). Baicalin suppresses lung cancer growth by targeting PDZ-binding kinase/T-LAK cell-originated protein kinase. Bioscience Reports, 39(4), BSR20181692.

Kruthika, B. S., Jain, R., Arivazhagan, A., Bharath, R. D., Yasha, T. C., Kondaiah, P., & Santosh, V. (2019). Transcriptome profiling reveals PDZ binding kinase as a novel biomarker in peritumoral brain zone of glioblastoma. Journal of neuro-oncology, 141, 315-325.

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For research use only. Not intended for any clinical use.

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