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Mouse Anti-PADI4 Recombinant Antibody (5B7) (CBMAB-P0644-YC)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
5B7
Antibody Isotype
IgG2a
Application
FC, WB

Basic Information

Immunogen
Human recombinant protein fragment correspongding to amino acids 299-588 of human PADI4(NP_036519) produced in E. coli
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
peptidyl arginine deiminase, type IV
Introduction
PADI4 is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response.
Entrez Gene ID
23569
UniProt ID
Q9UM07
Alternative Names
Peptidyl Arginine Deiminase 4; Peptidyl Arginine Deiminase, Type IV; Peptidyl Arginine Deiminase, Type V; Protein-Arginine Deiminase Type IV; EC 3.5.3.15; HL-60 PAD; PADI5; PAD4;
Function
Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance (PubMed:15339660, PubMed:15345777, PubMed:16567635, PubMed:21245532).
Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci) (PubMed:15339660, PubMed:15345777, PubMed:16567635, PubMed:21245532).
Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance (PubMed:15339660, PubMed:15345777, PubMed:16567635, PubMed:21245532).
Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci (PubMed:18209087).
Required for the formation of neutrophil extracellular traps (NETs); NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity).
Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription (PubMed:15345777).
Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1 (PubMed:15731352).
Biological Process
Cellular protein modification processManual Assertion Based On ExperimentTAS:ProtInc
Chromatin organizationISS:UniProtKB
Chromatin remodelingISS:UniProtKB
Histone citrullinationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H3-R26 citrullinationManual Assertion Based On ExperimentIDA:UniProtKB
Innate immune responseIEA:UniProtKB-KW
Nucleosome assemblyISS:UniProtKB
Protein citrullinationManual Assertion Based On ExperimentIDA:UniProtKB
Stem cell population maintenanceISS:UniProtKB
Cellular Location
Cytoplasm
Nucleus
Cytoplasmic granule
Cytoplasmic granules of eosinophils and neutrophils.
Involvement in disease
Rheumatoid arthritis (RA):
An inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.
PTM
Autocitrullination at Arg-372 and Arg-374 inactivates the enzyme.
More Infomation

Nava-Quiroz, K. J., Rojas-Serrano, J., Pérez-Rubio, G., Buendia-Roldan, I., Mejía, M., Fernández-López, J. C., ... & Falfán-Valencia, R. (2023). Molecular factors in PAD2 (PADI2) and PAD4 (PADI4) are associated with interstitial lung disease susceptibility in rheumatoid arthritis patients. Cells, 12(18), 2235.

Wang, Y., Song, X., Song, Y., Fang, K., & Chang, X. (2023). Investigating the cell membrane localization of PADI4 in breast cancer cells and inhibition of anti-PADI4 monoclonal antibody. Journal of Cancer Research and Clinical Oncology, 149(19), 17253-17268.

Indeglia, A., Leung, J. C., Miller, S. A., Leu, J. I. J., Dougherty, J. F., Clarke, N. L., ... & Murphy, M. E. (2023). An African-Specific Variant of TP5 3 Reveals PADI4 as a Regulator of p53-Mediated Tumor Suppression. Cancer discovery, 13(7), 1696-1719.

Neira, J. L., Araujo-Abad, S., Cámara-Artigas, A., Rizzuti, B., Abian, O., Giudici, A. M., ... & de Juan Romero, C. (2022). Biochemical and biophysical characterization of PADI4 supports its involvement in cancer. Archives of Biochemistry and Biophysics, 717, 109125.

Gu, W., Zhang, M., Gao, F., Niu, Y., Sun, L., Xia, H., ... & Du, G. (2022). Berberine regulates PADI4-related macrophage function to prevent lung cancer. International Immunopharmacology, 110, 108965.

Chang, X. T., Wu, H., Li, H. L., Li, H. L., & Zheng, Y. B. (2022). PADI4 promotes epithelial-mesenchymal transition (EMT) in gastric cancer via the upregulation of interleukin 8. Bmc Gastroenterology, 22(1), 25.

Lesbon, J. C. C., Garnica, T. K., Xavier, P. L. P., Rochetti, A. L., Reis, R. M., Müller, S., & Fukumasu, H. (2022). A Screening of Epigenetic Therapeutic Targets for Non-Small Cell Lung Cancer Reveals PADI4 and KDM6B as Promising Candidates. International Journal of Molecular Sciences, 23(19), 11911.

Wang, Y., Lyu, Y., Tu, K., Xu, Q., Yang, Y., Salman, S., ... & Semenza, G. L. (2021). Histone citrullination by PADI4 is required for HIF-dependent transcriptional responses to hypoxia and tumor vascularization. Science Advances, 7(35), eabe3771.

Liu, C., Tang, J., Li, C., Pu, G., Yang, D., & Chang, X. (2019). PADI4 stimulates esophageal squamous cell carcinoma tumor growth and up‐regulates CA9 expression. Molecular Carcinogenesis, 58(1), 66-75.

Liu, M., Qu, Y., Teng, X., Xing, Y., Li, D., Li, C., & Cai, L. (2019). PADI4‑mediated epithelial‑mesenchymal transition in lung cancer cells. Molecular medicine reports, 19(4), 3087-3094.

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For research use only. Not intended for any clinical use.

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