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Rat Anti-NT5E Recombinant Antibody (TY/23) (CBMAB-0998-LY)


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Summary

Host Animal
Rat
Specificity
Mouse
Clone
TY/23
Antibody Isotype
IgG2a
Application
Blocking

Basic Information

Specificity
Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
5'-Nucleotidase Ecto
Introduction
This gene encodes a membrane-bound nucleotidase that hydrolyzes extracellular nucleoside monophosphates. The encoded preproprotein undergoes proteolytic processing to generate to a functional, homodimeric enzyme that preferentially uses adenosine monophosphate as a substrate to generate free adenosine. Mice lacking the encoded protein exhibit a significantly reduced fall in stop flow pressure and superficial nephron glomerular filtration rate in response to a saturating increase of tubular perfusion flow. Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Entrez Gene ID
23959
UniProt ID
Q61503
Alternative Names
NT; Nt5; eNT; CD73; 5'-NT; AI447961; 2210401F01Rik; Nt5e
Function
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.
Biological Process
Adenosine biosynthetic processIEA:Ensembl
ADP catabolic processIEA:Ensembl
AMP catabolic processManual Assertion Based On ExperimentIBA:GO_Central
ATP metabolic processManual Assertion Based On ExperimentIDA:MGI
BiomineralizationManual Assertion Based On ExperimentIDA:MGI
Calcium ion homeostasisIEA:Ensembl
DNA metabolic processManual Assertion Based On ExperimentTAS:ProtInc
Leukocyte cell-cell adhesionManual Assertion Based On ExperimentIDA:CACAO
Negative regulation of inflammatory responseIEA:Ensembl
Response to ATPManual Assertion Based On ExperimentIDA:MGI
Response to inorganic substanceManual Assertion Based On ExperimentIDA:MGI
Cellular Location
Cell membrane
Involvement in disease
Calcification of joints and arteries (CALJA):
A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
More Infomation

Barge, S., Wu, A., Zhang, L., Robson, S. C., Olumi, A., Alper, S. L., ... & Yu, W. (2024). Role of ecto‐5′‐nucleotidase in bladder function. The FASEB Journal, 38(2), e23416.

Kordaß, T., Chao, T. Y., Osen, W., & Eichmüller, S. B. (2023). Novel microRNAs modulating ecto-5′-nucleotidase expression. Frontiers in Immunology, 14, 1199374.

Murakami, Y., Ando, M., Futamata, R., Horibe, T., Ueda, K., Kinoshita, M., & Kobayashi, T. (2022). Targeted deletion of ecto-5′-nucleotidase results in retention of inosine monophosphate content in postmortem muscle of medaka (Oryzias latipes). Scientific Reports, 12(1), 18588.

da Silva, F. C., de Melo Neto, A. B., Martins, C. A., de Sousa Cardoso, T. C., de Souza Gomes, M., de Araújo, T. G., & Fürstenau, C. R. (2022). Is the regulation by miRNAs of NTPDase1 and ecto-5’-nucleotidase genes involved with the different profiles of breast cancer subtypes?. Purinergic Signalling, 18(1), 123-133.

Alcedo, K. P., Bowser, J. L., & Snider, N. T. (2021). The elegant complexity of mammalian ecto-5′-nucleotidase (CD73). Trends in cell biology, 31(10), 829-842.

Shali, S., Yu, J., Zhang, X., Wang, X., Jin, Y., Su, M., ... & Zhou, P. (2019). Ecto‐5′‐nucleotidase (CD73) is a potential target of hepatocellular carcinoma. Journal of cellular physiology, 234(7), 10248-10259.

Minor, M., Alcedo, K. P., Battaglia, R. A., & Snider, N. T. (2019). Cell type-and tissue-specific functions of ecto-5′-nucleotidase (CD73). American Journal of Physiology-Cell Physiology, 317(6), C1079-C1092.

Hu, S., Meng, F., Yin, X., Cao, C., & Zhang, G. (2019). NT5E is associated with unfavorable prognosis and regulates cell proliferation and motility in gastric cancer. Bioscience reports, 39(5), BSR20190101.

Bertoni, A. P. S., Bracco, P. A., de Campos, R. P., Lutz, B. S., Assis-Brasil, B. M., de Souza Meyer, E. L., ... & Wink, M. R. (2019). Activity of ecto-5′-nucleotidase (NT5E/CD73) is increased in papillary thyroid carcinoma and its expression is associated with metastatic lymph nodes. Molecular and cellular endocrinology, 479, 54-60.

Kobarfard, F., Noroozi, R., Beirami, A. D., Taheri, M., & Ghafouri-Fard, S. (2019). Dys-regulation of peripheral transcript levels of ecto-5’-nucleotidase in multiple sclerosis patients. Human Antibodies, 27(3), 161-165.

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For research use only. Not intended for any clinical use.

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