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Mouse Anti-NT5E Recombinant Antibody (CBFYA-0173) (CBMAB-X0248-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYA-0173
Antibody Isotype
IgG1
Application
FC, IHC-Fr, FuncS, IF, IP, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% BSA
Concentration
0.1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
5'-Nucleotidase Ecto
Introduction
The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.
Entrez Gene ID
4907
UniProt ID
P21589
Alternative Names
5'-Nucleotidase Ecto; 5-Nucleotidase Ecto; Ecto-5-Nucleotidase; EC 3.1.3.5; 5-NT; NT5; NTE; 5-Nucleotidase, Ecto (CD73); Purine 5-Prime-Nucleotidase; 5 Nucleotidase (CD73)
Function
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.
Biological Process
Adenosine biosynthetic processIEA:Ensembl
ADP catabolic processIEA:Ensembl
AMP catabolic processManual Assertion Based On ExperimentIBA:GO_Central
ATP metabolic processManual Assertion Based On ExperimentIDA:MGI
BiomineralizationManual Assertion Based On ExperimentIDA:MGI
Calcium ion homeostasisIEA:Ensembl
DNA metabolic processManual Assertion Based On ExperimentTAS:ProtInc
Leukocyte cell-cell adhesionManual Assertion Based On ExperimentIDA:CACAO
Negative regulation of inflammatory responseIEA:Ensembl
Response to ATPManual Assertion Based On ExperimentIDA:MGI
Response to inorganic substanceManual Assertion Based On ExperimentIDA:MGI
Cellular Location
Cell membrane
Involvement in disease
Calcification of joints and arteries (CALJA):
A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
More Infomation

Barge, S., Wu, A., Zhang, L., Robson, S. C., Olumi, A., Alper, S. L., ... & Yu, W. (2024). Role of ecto‐5′‐nucleotidase in bladder function. The FASEB Journal, 38(2), e23416.

Kordaß, T., Chao, T. Y., Osen, W., & Eichmüller, S. B. (2023). Novel microRNAs modulating ecto-5′-nucleotidase expression. Frontiers in Immunology, 14, 1199374.

Murakami, Y., Ando, M., Futamata, R., Horibe, T., Ueda, K., Kinoshita, M., & Kobayashi, T. (2022). Targeted deletion of ecto-5′-nucleotidase results in retention of inosine monophosphate content in postmortem muscle of medaka (Oryzias latipes). Scientific Reports, 12(1), 18588.

da Silva, F. C., de Melo Neto, A. B., Martins, C. A., de Sousa Cardoso, T. C., de Souza Gomes, M., de Araújo, T. G., & Fürstenau, C. R. (2022). Is the regulation by miRNAs of NTPDase1 and ecto-5’-nucleotidase genes involved with the different profiles of breast cancer subtypes?. Purinergic Signalling, 18(1), 123-133.

Alcedo, K. P., Bowser, J. L., & Snider, N. T. (2021). The elegant complexity of mammalian ecto-5′-nucleotidase (CD73). Trends in cell biology, 31(10), 829-842.

Shali, S., Yu, J., Zhang, X., Wang, X., Jin, Y., Su, M., ... & Zhou, P. (2019). Ecto‐5′‐nucleotidase (CD73) is a potential target of hepatocellular carcinoma. Journal of cellular physiology, 234(7), 10248-10259.

Minor, M., Alcedo, K. P., Battaglia, R. A., & Snider, N. T. (2019). Cell type-and tissue-specific functions of ecto-5′-nucleotidase (CD73). American Journal of Physiology-Cell Physiology, 317(6), C1079-C1092.

Hu, S., Meng, F., Yin, X., Cao, C., & Zhang, G. (2019). NT5E is associated with unfavorable prognosis and regulates cell proliferation and motility in gastric cancer. Bioscience reports, 39(5), BSR20190101.

Bertoni, A. P. S., Bracco, P. A., de Campos, R. P., Lutz, B. S., Assis-Brasil, B. M., de Souza Meyer, E. L., ... & Wink, M. R. (2019). Activity of ecto-5′-nucleotidase (NT5E/CD73) is increased in papillary thyroid carcinoma and its expression is associated with metastatic lymph nodes. Molecular and cellular endocrinology, 479, 54-60.

Kobarfard, F., Noroozi, R., Beirami, A. D., Taheri, M., & Ghafouri-Fard, S. (2019). Dys-regulation of peripheral transcript levels of ecto-5’-nucleotidase in multiple sclerosis patients. Human Antibodies, 27(3), 161-165.

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For research use only. Not intended for any clinical use.

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