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Mouse Anti-NR5A2 Recombinant Antibody (CBYJL-223) (CBMAB-L0312-YJ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJL-223
Antibody Isotype
IgG2a
Application
WB, IP

Basic Information

Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
NUCLEAR RECEPTOR SUBFAMILY 5 GROUP A MEMBER 2
Introduction
NR5A2 is a DNA-binding zinc finger transcription factor and is a member of the fushi tarazu factor-1 subfamily of orphan nuclear receptors. NR5A2 is involved in the expression of genes for hepatitis B virus and cholesterol biosynthesis, and may be an important regulator of embryonic development.
Entrez Gene ID
2494
UniProt ID
O00482
Alternative Names
Nuclear Receptor Subfamily 5 Group A Member 2; CYP7A Promoter-Binding Factor; LRH-1; FTF; B1F; CPF; B1-Binding Factor; Hepatocyte Transcription Factor Which Activates Enhancer II Of Hepatitis B Virus; Nuclear Receptor Subfamily 5; Group A; Member 2; Fetoprotein-Alpha 1 (AFP) Transcription Factor; Liver Nuclear Receptor Homolog-1 Variant 2; Alpha-1-Fetoprotein Transcription Factor; Hepatocytic Transcription Factor HB1F-3
Function
Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex (PubMed:20159957).
Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. Activates the transcription of CYP2C38 (By similarity).
Biological Process
Bile acid metabolic processIEA:Ensembl
Calcineurin-mediated signalingIEA:Ensembl
Cellular response to leukemia inhibitory factorIEA:Ensembl
Cholesterol homeostasisIEA:Ensembl
Embryo development ending in birth or egg hatchingManual Assertion Based On ExperimentTAS:UniProtKB
Epithelial cell differentiationIEA:Ensembl
Homeostatic process1 PublicationNAS:UniProtKB
Hormone-mediated signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Pancreas morphogenesisIEA:Ensembl
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of viral genome replicationManual Assertion Based On ExperimentIDA:BHF-UCL
Regulation of cell population proliferationIEA:Ensembl
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Tissue developmentManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
PTM
Sumoylated by SUMO1 at Lys-270 during the hepatic acute phase response, leading to promote interaction with GPS2 and prevent N-Cor corepressor complex dissociation.
More Infomation

Zheng, Q., Tang, J., Aicher, A., Bou Kheir, T., Sabanovic, B., Ananthanarayanan, P., ... & Heeschen, C. (2023). Inhibiting NR5A2 targets stemness in pancreatic cancer by disrupting SOX2/MYC signaling and restoring chemosensitivity. Journal of Experimental & Clinical Cancer Research, 42(1), 323.

Festuccia, N., Vandormael-Pournin, S., Chervova, A., Geiselman, A., Langa-Vives, F., Coux, R. X., ... & Navarro, P. (2023). Nr5a2 is essential for morula development. bioRxiv, 2023-01.

Lai, F., Li, L., Hu, X., Liu, B., Zhu, Z., Liu, L., ... & Xie, W. (2023). NR5A2 connects zygotic genome activation to the first lineage segregation in totipotent embryos. Cell Research, 33(12), 952-966.

Gassler, J., Kobayashi, W., Gáspár, I., Ruangroengkulrith, S., Mohanan, A., Gómez Hernández, L., ... & Tachibana, K. (2022). Zygotic genome activation by the totipotency pioneer factor Nr5a2. Science, 378(6626), 1305-1315.

Guo, F., Zhou, Y., Guo, H., Ren, D., Jin, X., & Wu, H. (2021). NR5A2 transcriptional activation by BRD4 promotes pancreatic cancer progression by upregulating GDF15. Cell Death Discovery, 7(1), 78.

Tsampoula, M., Tarampoulous, I., Antoniadou, I., Koutmani, Y., Gkikas, D., Vekrellis, K., & Politis, P. K. (2021). Nuclear receptor NR5A2 promotes neuronal identity in the adult hippocampus. Molecular Neurobiology, 58, 1952-1962.

Festuccia, N., Owens, N., Chervova, A., Dubois, A., & Navarro, P. (2021). The combined action of Esrrb and Nr5a2 is essential for murine naive pluripotency. Development, 148(17), dev199604.

Gkikas, D., Stellas, D., Polissidis, A., Manolakou, T., Kokotou, M. G., Kokotos, G., & Politis, P. K. (2021). Nuclear receptor NR5A2 negatively regulates cell proliferation and tumor growth in nervous system malignancies. Proceedings of the National Academy of Sciences, 118(39), e2015243118.

Yang, Q., Deng, L., Li, J., Miao, P., Liu, W., & Huang, Q. (2020). NR5A2 promotes cell growth and resistance to temozolomide through regulating notch signal pathway in glioma. OncoTargets and therapy, 10231-10244.

Ye, T., Li, J., Sun, Z., Liu, Y., Kong, L., Zhou, S., ... & Xing, H. R. (2019). Nr5a2 promotes cancer stem cell properties and tumorigenesis in nonsmall cell lung cancer by regulating Nanog. Cancer medicine, 8(3), 1232-1245.

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For research use only. Not intended for any clinical use.

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