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Rabbit Anti-NDRG1 Recombinant Antibody (D6C2) (CBMAB-N1576-WJ)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
D6C2
Antibody Isotype
IgG
Application
WB

Basic Information

Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
N-Myc Downstream Regulated 1
Introduction
This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
Entrez Gene ID
Human10397
Mouse17988
Rat299923
UniProt ID
HumanQ92597
MouseQ62433
RatQ6JE36
Alternative Names
N-Myc Downstream Regulated 1; Reducing Agents And Tunicamycin-Responsive Protein; N-Myc Downstream-Regulated Gene 1 Protein; Nickel-Specific Induction Protein Cap43; Differentiation-Related Gene 1 Protein; DRG-1; RIT42; CAP43; DRG1; Rtp; Protein Regulated By Oxygen-1;
Function
Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy.
Biological Process
Cellular response to hypoxia Source: UniProtKB
DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
Mast cell activation Source: Ensembl
Negative regulation of cell population proliferation Source: Ensembl
Peripheral nervous system myelin maintenance Source: Ensembl
Response to metal ion Source: ProtInc
Signal transduction Source: GO_Central
Cellular Location
Nucleus
Cytoskeleton
centrosome
cytosol
Plasma membrane
Cell membrane
Note: Mainly cytoplasmic but differentially localized to other regions. Associates with the plasma membrane in intestinal epithelia and lactating mammary gland. Translocated to the nucleus in a p53/TP53-dependent manner. In prostate epithelium and placental chorion, located in both the cytoplasm and in the nucleus. No nuclear localization in colon epithelium cells. In intestinal mucosa, prostate and renal cortex, located predominantly adjacent to adherens junctions. Cytoplasmic with granular staining in proximal tubular cells of the kidney and salivary gland ducts. Recruits to the membrane of recycling/sorting and late endosomes via binding to phosphatidylinositol 4-phosphate. Associates with microtubules. Colocalizes with TUBG1 in the centrosome. Cytoplasmic location increased with hypoxia. Phosphorylated form found associated with centromeres during S-phase of mitosis and with the plasma membrane.
Involvement in disease
Charcot-Marie-Tooth disease 4D (CMT4D):
A recessive demyelinating form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Demyelinating neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4.
PTM
Under stress conditions, phosphorylated in the C-terminal on many serine and threonine residues. Phosphorylated in vitro by PKA. Phosphorylation enhanced by increased intracellular cAMP levels. Homocysteine induces dephosphorylation. Phosphorylation by SGK1 is cell cycle dependent.
More Infomation

Deng, Z., & Richardson, D. R. (2023). The myc family and the metastasis suppressor NDRG1: targeting key molecular interactions with innovative therapeutics. Pharmacological Reviews, 75(5), 1007-1035.

Zhao, X., & Richardson, D. R. (2023). The role of the NDRG1 in the pathogenesis and treatment of breast cancer. Biochimica et Biophysica Acta (BBA)-Reviews on Cancer, 188871.

Zhang, G., Qin, Q., Zhang, C., Sun, X., Kazama, K., Yi, B., ... & Sun, J. (2023). NDRG1 signaling is essential for endothelial inflammation and vascular remodeling. Circulation Research, 132(3), 306-319.

Joshi, V., Lakhani, S. R., & McCart Reed, A. E. (2022). NDRG1 in Cancer: A Suppressor, Promoter, or Both?. Cancers, 14(23), 5739.

Villodre, E. S., Hu, X., Eckhardt, B. L., Larson, R., Huo, L., Yoon, E. C., ... & Debeb, B. G. (2022). NDRG1 in aggressive breast cancer progression and brain metastasis. JNCI: Journal of the National Cancer Institute, 114(4), 579-591.

Chekmarev, J., Azad, M. G., & Richardson, D. R. (2021). The oncogenic signaling disruptor, NDRG1: Molecular and cellular mechanisms of activity. Cells, 10(9), 2382.

Yang, G., Huang, L., Jia, H., Aikemu, B., Zhang, S., Shao, Y., ... & Ma, J. (2021). NDRG1 enhances the sensitivity of cetuximab by modulating EGFR trafficking in colorectal cancer. Oncogene, 40(41), 5993-6006.

Lim, S. C., Geleta, B., Maleki, S., Richardson, D. R., & Kovačević, Ž. (2021). The metastasis suppressor NDRG1 directly regulates androgen receptor signaling in prostate cancer. Journal of Biological Chemistry, 297(6).

Park, K. C., Paluncic, J., Kovacevic, Z., & Richardson, D. R. (2020). Pharmacological targeting and the diverse functions of the metastasis suppressor, NDRG1, in cancer. Free Radical Biology and Medicine, 157, 154-175.

Villodre, E. S., Gong, Y., Hu, X., Huo, L., Yoon, E. C., Ueno, N. T., ... & Debeb, B. G. (2020). NDRG1 expression is an independent prognostic factor in inflammatory breast cancer. Cancers, 12(12), 3711.

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For research use only. Not intended for any clinical use.

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