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Mouse Anti-MTHFD1L Recombinant Antibody (1E8) (CBMAB-A5661-LY)


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Summary

Host Animal
Mouse
Specificity
Human, Rat
Clone
1E8
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
MTHFD1L (NP_056255, 801 a.a. ~ 899 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1 Like
Introduction
One-carbon substituted forms of tetrahydrofolate (THF) are involved in the de novo synthesis of purines and thymidylate and support cellular methylation reactions through the regeneration of methionine from homocysteine. MTHFD1L is an enzyme involved in THF synthesis in mitochondria (Christensen et al., 2005 [PubMed 15611115]).[supplied by OMIM
Entrez Gene ID
Human25902
Rat361472
UniProt ID
HumanQ6UB35
RatB2GUZ3
Alternative Names
DKFZp586G1517; FLJ21145; FTHFSDC1; MTC1THFS; dJ292B18.2
Function
May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism complementing thus the enzymatic activities of MTHFD2.
Biological Process
10-formyltetrahydrofolate biosynthetic process Source: BHF-UCL
Embryonic neurocranium morphogenesis Source: BHF-UCL
Embryonic viscerocranium morphogenesis Source: BHF-UCL
Folic acid-containing compound metabolic process Source: BHF-UCL
Formate metabolic process Source: BHF-UCL
Neural tube closure Source: BHF-UCL
Tetrahydrofolate interconversion Source: GO_Central
Cellular Location
Mitochondrion
More Infomation

Green, A. C., Marttila, P., Kiweler, N., Chalkiadaki, C., Wiita, E., Cookson, V., ... & Meiser, J. (2023). Formate overflow drives toxic folate trapping in MTHFD1 inhibited cancer cells. Nature Metabolism, 5(4), 642-659.

Rao, K., Zheng, K., Zhao, Q., He, J., Zhou, B., Hou, G., ... & Xia, Q. (2023). The negative effect of G1958A polymorphism on MTHFD1 protein stability and HCC growth. Cellular Oncology, 46(3), 735-744.

Meng, Q., Lu, Y. X., Wei, C., Wang, Z. X., Lin, J. F., Liao, K., ... & Ju, H. Q. (2022). Arginine methylation of MTHFD1 by PRMT5 enhances anoikis resistance and cancer metastasis. Oncogene, 41(32), 3912-3924.

Vohra, M., Adhikari, P., CD, S., Nagri, S. K., Umakanth, S., Satyamoorthy, K., & Rai, P. S. (2020). CpG-SNP site methylation regulates allele-specific expression of MTHFD1 gene in type 2 diabetes. Laboratory Investigation, 100(8), 1090-1101.

Bidla, G., Watkins, D., Chéry, C., Froese, D. S., Ells, C., Kerachian, M., ... & Rosenblatt, D. S. (2020). Biochemical analysis of patients with mutations in MTHFD1 and a diagnosis of methylenetetrahydrofolate dehydrogenase 1 deficiency. Molecular Genetics and Metabolism, 130(3), 179-182.

Xu, M., Li, J., Chen, X., Han, L., Li, L., & Liu, Y. (2019). MTHFD1 promoter hypermethylation increases the risk of hypertension. Clinical and Experimental Hypertension, 41(5), 422-427.

Sdelci, S., Rendeiro, A. F., Rathert, P., You, W., Lin, J. M. G., Ringler, A., ... & Kubicek, S. (2019). MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation. Nature genetics, 51(6), 990-998.

Yu, H., Wang, H., Xu, H. R., Zhang, Y. C., Yu, X. B., Wu, M. C., ... & Cong, W. M. (2019). Overexpression of MTHFD1 in hepatocellular carcinoma predicts poorer survival and recurrence. Future Oncology, 15(15), 1771-1780.

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For research use only. Not intended for any clinical use.

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