Mouse Anti-MLF2 (AA 1-248) Recombinant Antibody (CBFYM-2285) (CBMAB-M2470-FY)

See all MLF2 antibodies

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Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYM-2285

Antibody Isotype

IgG1, k

Application

S-ELISA, IHC-P, ELISA, WB-Re, WB-Tr

Basic Information

Immunogen

Full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG1, k

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-248

Target

Full Name

Myeloid Leukemia Factor 2

Introduction

MLF2 is a Protein Coding gene. Diseases associated with MLF2 include Myeloid Leukemia and Leukemia. An important paralog of this gene is MLF1.

Entrez Gene ID

8079

UniProt ID

Q15773

Alternative Names

Myeloid Leukemia Factor 2; Myelodysplasia-Myeloid Leukemia Factor 2; NTN4

Biological Process

Regulation of transcription, DNA-templated Source: GO_Central

Cellular Location

Nucleus
Cytoplasm

More Infomation

References

Fang, D., Hu, H., Zhao, K., Xu, A., Yu, C., Zhu, Y., ... & Mei, Y. (2023). MLF2 Negatively Regulates P53 and Promotes Colorectal Carcinogenesis. Advanced Science, 10(26), 2303336.

Ogunleye, A. J., Romanova, E., & Medvedeva, Y. A. (2021). Genome-wide regulation of CpG methylation by ecCEBPα in acute myeloid leukemia. F1000Research, 10.

Yang, J., Cao, D., Zhang, Y., Ou, R., Yin, Z., Liu, Y., ... & Fei, J. (2020). The role of phosphorylation of MLF2 at serine 24 in BCR-ABL leukemogenesis. Cancer Gene Therapy, 27(1-2), 98-107.

Huang, D., Liu, C., Sun, X., Sun, X., Qu, Y., Tang, Y., ... & Tong, T. (2020). CRL4DCAF8 and USP11 oppositely regulate the stability of myeloid leukemia factors (MLFs). Biochemical and Biophysical Research Communications, 529(2), 127-132.

Rampello, A. J., Laudermilch, E., Vishnoi, N., Prophet, S. M., Shao, L., Zhao, C., ... & Schlieker, C. (2020). Torsin ATPase deficiency leads to defects in nuclear pore biogenesis and sequestration of MLF2. Journal of Cell Biology, 219(6).

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Antibody Pairs

MLF2 Matched Antibody Pair (738)

MLF2 Matched Antibody Pair (739)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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