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Mouse Anti-MDH1 Recombinant Antibody (CBFYM-1942) (CBMAB-M2116-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-1942
Antibody Isotype
IgG2a, k
Application
ELISA, IHC-P, WB

Basic Information

Immunogen
Recombinant protein encoding amino acids 101 to 193 of human MDH1
Specificity
Human
Antibody Isotype
IgG2a, k
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Concentration
0.5 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
MDH1
Introduction
This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6.
Entrez Gene ID
4190
UniProt ID
P40925
Alternative Names
Malate Dehydrogenase 1; Malate Dehydrogenase 1, NAD (Soluble); Cytosolic Malate Dehydrogenase; Diiodophenylpyruvate Reductase; EC 1.1.1.37; MDHA; Epididymis Secretory Protein Li 32; Malate Dehydrogenase, Cytoplasmic
Function
Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH (PubMed:3052244).

Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation (PubMed:31538237).
Biological Process
Malate metabolic process Source: GO_Central
NADH metabolic process Source: GO_Central
Oxaloacetate metabolic process Source: GO_Central
Tricarboxylic acid cycle Source: GO_Central
Cellular Location
Cytoplasm
Involvement in disease
Developmental and epileptic encephalopathy 88 (DEE88):
A form of epileptic encephalopathy, a heterogeneous group of early-onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. DEE88 is an autosomal recessive severe form characterized by global developmental delay, epilepsy, and progressive microcephaly.
PTM
ISGylated.
Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.
More Infomation

Kwon, H. J., Hahn, K. R., Kang, M. S., Choi, J. H., Moon, S. M., Yoon, Y. S., ... & Kim, D. W. (2023). Tat-malate dehydrogenase fusion protein protects neurons from oxidative and ischemic damage by reduction of reactive oxygen species and modulation of glutathione redox system. Scientific Reports, 13(1), 5653.

Thomas, M. J., Cassidy, E. R., Robinson, D. S., & Walstrom, K. M. (2022). Kinetic characterization and thermostability of C. elegans cytoplasmic and mitochondrial malate dehydrogenases. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1870(1), 140722.

Zhu, Q., Zhou, H., Wu, L., Lai, Z., Geng, D., Yang, W., ... & Yi, W. (2022). O-GlcNAcylation promotes pancreatic tumor growth by regulating malate dehydrogenase 1. Nature chemical biology, 18(10), 1087-1095.

Imran, M., Munir, M. Z., Ialhi, S., Abbas, F., Younus, M., Ahmad, S., ... & Shafiq, S. (2022). Identification and Characterization of Malate Dehydrogenases in Tomato (Solanum lycopersicum L.). International Journal of Molecular Sciences, 23(17), 10028.

McCue, W. M., & Finzel, B. C. (2021). Structural characterization of the human cytosolic malate dehydrogenase I. ACS omega, 7(1), 207-214.

Gu, H., Chen, C., Hao, X., Su, N., Huang, D., Zou, Y., ... & Zheng, J. (2020). MDH1-mediated malate-aspartate NADH shuttle maintains the activity levels of fetal liver hematopoietic stem cells. Blood, The Journal of the American Society of Hematology, 136(5), 553-571.

Nan, N., Wang, J., Shi, Y., Qian, Y., Jiang, L., Huang, S., ... & Xu, Z. Y. (2020). Rice plastidial NAD‐dependent malate dehydrogenase 1 negatively regulates salt stress response by reducing the vitamin B6 content. Plant Biotechnology Journal, 18(1), 172-184.

Jin, J., Huang, S., & Pan, J. (2019). A Novel Role for Malate Dehydrogenase 1 in Acute Myeloid Leukemia Progression. Blood, 134, 5048.

Broeks, M. H., Shamseldin, H. E., Alhashem, A., Hashem, M., Abdulwahab, F., Alshedi, T., ... & Alkuraya, F. S. (2019). MDH1 deficiency is a metabolic disorder of the malate–aspartate shuttle associated with early onset severe encephalopathy. Human genetics, 138, 1247-1257.

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For research use only. Not intended for any clinical use.

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