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Mouse Anti-MDH1 Recombinant Antibody (1D2) (CBMAB-A5357-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
1D2
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
MDH1 (AAH01484.1, 1 a.a. ~ 334 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
MDH1
Introduction
Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. [provided by RefSeq]
Entrez Gene ID
4190
UniProt ID
P40925
Alternative Names
MDH-s; MDHA; MGC:1375; MOR2
Function
Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH (PubMed:3052244).

Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation (PubMed:31538237).
Biological Process
Malate metabolic process Source: GO_Central
NADH metabolic process Source: GO_Central
Oxaloacetate metabolic process Source: GO_Central
Tricarboxylic acid cycle Source: GO_Central
Cellular Location
Cytoplasm
Involvement in disease
Developmental and epileptic encephalopathy 88 (DEE88):
A form of epileptic encephalopathy, a heterogeneous group of early-onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. DEE88 is an autosomal recessive severe form characterized by global developmental delay, epilepsy, and progressive microcephaly.
PTM
ISGylated.
Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.
More Infomation

Kwon, H. J., Hahn, K. R., Kang, M. S., Choi, J. H., Moon, S. M., Yoon, Y. S., ... & Kim, D. W. (2023). Tat-malate dehydrogenase fusion protein protects neurons from oxidative and ischemic damage by reduction of reactive oxygen species and modulation of glutathione redox system. Scientific Reports, 13(1), 5653.

Thomas, M. J., Cassidy, E. R., Robinson, D. S., & Walstrom, K. M. (2022). Kinetic characterization and thermostability of C. elegans cytoplasmic and mitochondrial malate dehydrogenases. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1870(1), 140722.

Zhu, Q., Zhou, H., Wu, L., Lai, Z., Geng, D., Yang, W., ... & Yi, W. (2022). O-GlcNAcylation promotes pancreatic tumor growth by regulating malate dehydrogenase 1. Nature chemical biology, 18(10), 1087-1095.

Imran, M., Munir, M. Z., Ialhi, S., Abbas, F., Younus, M., Ahmad, S., ... & Shafiq, S. (2022). Identification and Characterization of Malate Dehydrogenases in Tomato (Solanum lycopersicum L.). International Journal of Molecular Sciences, 23(17), 10028.

McCue, W. M., & Finzel, B. C. (2021). Structural characterization of the human cytosolic malate dehydrogenase I. ACS omega, 7(1), 207-214.

Gu, H., Chen, C., Hao, X., Su, N., Huang, D., Zou, Y., ... & Zheng, J. (2020). MDH1-mediated malate-aspartate NADH shuttle maintains the activity levels of fetal liver hematopoietic stem cells. Blood, The Journal of the American Society of Hematology, 136(5), 553-571.

Nan, N., Wang, J., Shi, Y., Qian, Y., Jiang, L., Huang, S., ... & Xu, Z. Y. (2020). Rice plastidial NAD‐dependent malate dehydrogenase 1 negatively regulates salt stress response by reducing the vitamin B6 content. Plant Biotechnology Journal, 18(1), 172-184.

Jin, J., Huang, S., & Pan, J. (2019). A Novel Role for Malate Dehydrogenase 1 in Acute Myeloid Leukemia Progression. Blood, 134, 5048.

Broeks, M. H., Shamseldin, H. E., Alhashem, A., Hashem, M., Abdulwahab, F., Alshedi, T., ... & Alkuraya, F. S. (2019). MDH1 deficiency is a metabolic disorder of the malate–aspartate shuttle associated with early onset severe encephalopathy. Human genetics, 138, 1247-1257.

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For research use only. Not intended for any clinical use.

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