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Mouse Anti-MAD2L1 (AA 27-172) Recombinant Antibody (CBFYM-1256) (CBMAB-M1415-FY)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYM-1256
Antibody Isotype
IgG2a
Application
WB, IF, IHC

Basic Information

Immunogen
Human MAD2 aa. 27-172
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
0.25 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 27-172

Target

Full Name
Mitotic Arrest Deficient 2 Like 1
Introduction
MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14.
Entrez Gene ID
Human4085
Mouse56150
Rat297176
UniProt ID
HumanQ13257
MouseQ9Z1B5
RatD4ACM2
Alternative Names
Mitotic Arrest Deficient 2 Like 1; MAD2 (Mitotic Arrest Deficient, Yeast, Homolog)-Like 1; Mitotic Arrest Deficient 2-Like Protein 1; MAD2-Like Protein 1; HSMAD2; MAD2; Mitotic Spindle Assembly Checkpoint Protein MAD2A; Mitotic Arrest Deficient, Yeast, Homolog-Like 1; MAD2 Mitotic Arrest Deficient-Like 1
Function
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate (PubMed:29162720, PubMed:15024386).
In the closed conformation (C-MAD2) forms a heterotetrameric complex with MAD1L1 at unattached kinetochores during prometaphase, the complex recruits open conformation molecules of MAD2L1 (O-MAD2) and then promotes the conversion of O-MAD2 to C-MAD2 (PubMed:29162720).
Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate (PubMed:10700282, PubMed:11804586, PubMed:15024386).
Biological Process
Cell divisionIEA:UniProtKB-KW
Establishment of centrosome localizationManual Assertion Based On ExperimentIMP:UniProtKB
Establishment of mitotic spindle orientationManual Assertion Based On ExperimentIMP:UniProtKB
Mitotic sister chromatid segregationIEA:Ensembl
Mitotic spindle assembly checkpoint signalingManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of mitotic cell cycleManual Assertion Based On ExperimentIMP:MGI
Negative regulation of protein catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of ubiquitin protein ligase activityManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of ubiquitin-protein transferase activityManual Assertion Based On ExperimentIDA:ComplexPortal
Positive regulation of mitotic cell cycle spindle assembly checkpointManual Assertion Based On Experiment
Cellular Location
Nucleus
Chromosome, centromere, kinetochore
Cytoplasm
Cytoplasm, cytoskeleton, spindle pole
Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD. Sequestered to the cytoplasm upon interaction with isoform 3 of MAD1L1 (PubMed:19010891).
PTM
Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20. Phosphorylated by NEK2.
More Infomation

Li, Q., Tong, D., Jing, X., Ma, P., Li, F., Jiang, Q., ... & Zhang, M. (2023). MAD2L1 is transcriptionally regulated by TEAD4 and promotes cell proliferation and migration in colorectal cancer. Cancer Gene Therapy, 30(5), 727-737.

Wang, X., Yu, J., Yan, J., Peng, K., & Zhou, H. (2022). Single-cell sequencing reveals MYC targeting gene MAD2L1 is associated with prostate cancer bone metastasis tumor dormancy. BMC urology, 22(1), 37.

Chen, Q., Yang, S., Zhang, Y., Li, B., Xu, H., & Zuo, S. (2022). Identification of MAD2L1 as a potential biomarker in hepatocellular carcinoma via comprehensive bioinformatics analysis. BioMed Research International, 2022.

Ding, X., Fu, Q., Chen, W., Chen, L., Zeng, Q., Zhang, S., & He, L. (2022). Targeting of MAD2L1 by miR‐515‐5p involves the regulation of cell cycle arrest and apoptosis of colorectal cancer cells. Cell biology international, 46(5), 840-848.

Gao, Y., Liu, Y., Sun, L., Ouyang, X., Zhu, C., & Qin, X. (2021). MAD2L1 functions as a novel diagnostic and predictive biomarker in cholangiocarcinoma. Genetic Testing and Molecular Biomarkers, 25(11), 685-695.

Xia, T., Meng, L., Zhao, Z., Li, Y., Wen, H., Sun, H., ... & Liu, C. (2021). Bioinformatics prediction and experimental verification identify MAD2L1 and CCNB2 as diagnostic biomarkers of rhabdomyosarcoma. Cancer Cell International, 21, 1-20.

Busacca, S., O'Regan, L., Singh, A., Sharkey, A. J., Dawson, A. G., Dzialo, J., ... & Fennell, D. A. (2021). BRCA1/MAD2L1 deficiency disrupts the spindle assembly checkpoint to confer vinorelbine resistance in mesothelioma. Molecular cancer therapeutics, 20(2), 379-388.

Lu, S., Sun, C., Chen, H., Zhang, C., Li, W., Wu, L., ... & Zhang, X. (2020). Bioinformatics analysis and validation identify CDK1 and MAD2L1 as prognostic markers of rhabdomyosarcoma. Cancer Management and Research, 12123-12136.

Li, J., He, X., Wu, X., Liu, X., Huang, Y., & Gong, Y. (2020). miR-139-5p inhibits lung adenocarcinoma cell proliferation, migration, and invasion by targeting MAD2L1. Computational and Mathematical Methods in Medicine, 2020.

Wang, Y., Wang, F., He, J., Du, J., Zhang, H., Shi, H., ... & Zhang, J. (2019). miR-30a-3p targets MAD2L1 and regulates proliferation of gastric cancer cells. OncoTargets and therapy, 11313-11324.

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For research use only. Not intended for any clinical use.

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