MAD2L1

MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008]

Mitotic Arrest Deficient 2 Like 1

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate (PubMed:29162720, PubMed:15024386).
In the closed conformation (C-MAD2) forms a heterotetrameric complex with MAD1L1 at unattached kinetochores during prometaphase, the complex recruits open conformation molecules of MAD2L1 (O-MAD2) and then promotes the conversion of O-MAD2 to C-MAD2 (PubMed:29162720).
Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate (PubMed:10700282, PubMed:11804586, PubMed:15024386).

Cell divisionIEA:UniProtKB-KW
Establishment of centrosome localizationManual Assertion Based On ExperimentIMP:UniProtKB
Establishment of mitotic spindle orientationManual Assertion Based On ExperimentIMP:UniProtKB
Mitotic sister chromatid segregationIEA:Ensembl
Mitotic spindle assembly checkpoint signalingManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of mitotic cell cycleManual Assertion Based On ExperimentIMP:MGI
Negative regulation of protein catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of ubiquitin protein ligase activityManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of ubiquitin-protein transferase activityManual Assertion Based On ExperimentIDA:ComplexPortal
Positive regulation of mitotic cell cycle spindle assembly checkpointManual Assertion Based On Experiment

Nucleus
Chromosome, centromere, kinetochore
Cytoplasm
Cytoplasm, cytoskeleton, spindle pole
Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD. Sequestered to the cytoplasm upon interaction with isoform 3 of MAD1L1 (PubMed:19010891).

Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20. Phosphorylated by NEK2.